Biolegend, 537002, Purified anti-human VEGF Antibody, 500μg

VEGF (also known as VEGF-A) is a signaling protein with strong vascular permeability activity that stimulates the formation of new blood vessels during the processes of vasculogenesis and angiogenesis. It is a member of the vascular endothelial growth factor family, which in mammals is comprised of VEGF-A, VEGF-B, VEGF-C, VEGF-D, and PlGF (placental growth factor). There are multiple isoforms of VEGF that result from alternative splicing of mRNA. VEGF binds and activates two tyrosine kinase receptors, VEGFR1 (Flt-1) and VEGFR2 (KDR/Flk-1), through which VEGF exerts its mitogenic effects. VEGF is highly expressed in solid tumors of breast, lung, renal, colorectal, and hepatic origin. Additionally, it significantly contributes to ascites tumor formation. Beyond cancer, VEGF has also been associated with multiple pathogenic conditions including rheumatoid arthritis, age-related macular degeneration, Crow-Fukase syndrome, sclerosis and Alzheimer’s disease.
500μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Recombinant protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: ELISA Capture - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by ELISA assay. For ELISA Capture applications, a concentration range of 1 - 4 µg/mL is recommended. To obtain a linear standard curve, serial dilutions of VEGF recombinant protein ranging from 23.4 to 1,500 pg/mL is recommended for each ELISA plate. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: The purified A15136H antibody is useful as the capture antibody in a sandwich ELISA assay, when used in conjunction with the biotinylated Poly5225 antibody (Cat. No. 522503) as the detection antibody and Recombinant Human VEGF (ELISA Std.) (Cat. No. 560309)
RRID: AB_2783345 (BioLegend Cat. No. 537002)
Structure: Homodimer
Distribution: Widely expressed
Function: VEGFA is a key player in vasculogenesis, the formation of blood vessels from progenitor cells, as well as angiogenesis. The expression of the VEGFA gene is upregulated via hypoxia, estrogen, and NF-κB pathways. In addition VEGF is upregulated by PDGF-BB, P1GF, TGFβ1, IGF1, FGFs, HGF, TNFα, and IL-1. VEGFA induces proliferation and cell migration in endothelial cells and plays important roles during wound healing. Also, VEGF regulates hematopoietic stem cell survival.
Interaction: VEGFA interacts with vascular endothelial cells and monocytes/macrophages, which express VEGFR1. This interaction induces proliferation of endothelial cells and stimulates migration of monocytes/macrophages
Ligand/Receptor: Vascular endothelial growth factor (VEGF)-A binds and activates two tyrosine kinase receptors, VEGFR1 (Flt-1) and VEGFR2 (KDR/Flk-1)
Molecular Family: Cytokines/Chemokines
Antigen References: Conn G, et al. 1990. Proc. Natl. Acad. Sci USA 87:1323. Gerber H, et al. 2002. Nature 417:954. Shibuya M. 2006. J. Biochem. Mol. Biol. 39:469. Shibuya M. 2008. BMB Rep. 41:278. Monaghan-Benson E, et al. 2010. The American Journal of Pathology 177:2091. Koch S and Claesson-Welsh L. 2012. Cold Spring Harb Perspect Med. 2:a006502.
Gene ID: 7422
UniProt: View information about VEGF on UniProt.org
Clone: A15136H
Regulatory Status: RUO
Other Names: VEGFA, VEGF-A, VPF, MVCD1, vascular endothelial growth factor
Isotype: Mouse IgG2b, κ