Biolegend, 537602, Purified anti-human CCL13 (MCP-4) Antibody, 500μg

Human CCL13 (MCP-4) was initially identified in a library constructed from human fetal mRNA. CCL13 shares a high similarity with CCL2 (MCP-1, 61%), CCL7 (MCP-3, 58%), and CCL8 (MCP-2, 55%). CCL13 is constitutively expressed at high levels in the small intestine, colon, and lung. Original data showed that CCL13 was less effective than MCP-1 and MCP-3 in monocyte and lymphocyte chemoattraction, but equivalent to eotaxin as a chemoattractant for eosinophils. Chemokines are very important in the inflammatory process, initially in the influx of PMN by CXC chemokines and subsequently chemoattracting monocytes where the MCPs participate, including CCL13. The bioactivity of pro-inflammatory chemokines is regulated by MMPs in order to regulate the influx of different cell subpopulations. In the case of CCL13, it is inactivated by MMP-12 by truncation at position 4-5 generating a CCR antagonist and decreasing the arrival of monocytes. CCL13 binds to CCR2, and this receptor also binds to CCL2, CCL8, CCL7, CCL11, CCL24, and CCL26. CCL13 is involved in inflammatory diseases such as rheumatoid arthritis (RA), atherosclerosis, and asthma. High levels of CCL13 have been detected in the synovial fluid of patients with RA, in serum of asthmatic patients, and in plasma of patients with symptomatic carotid atherosclerosis.
500μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Recombinant protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: ELISA Capture - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by ELISA assay. For ELISA Capture applications, a concentration range of 0.4 - 3 µg/mL is recommended. To obtain a linear standard curve, serial dilutions of CCL13 (MCP4) recombinant protein ranging from 3.9 to 250 pg/mL is recommended for each ELISA plate. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: The pure 3G4 (Cat. No. 537602) antibody is useful as the capture antibody in a sandwich ELISA assay, paired with biotinylated 8C12 (Cat. No. 537704) and Lyophilized CCL13 (MCP4) (Cat. No. 587509).
RRID: AB_2783351 (BioLegend Cat. No. 537602)
Structure: Chemokine
Distribution: Epithelial cells, endothelial cells, fibroblasts, chondrocytes, macrophages
Function: CCL13 chemoattract monocytes, eosinophils, CD4, and CD8 T cells. CCL13 induces histamine release from IL-3-primed peripheral blood basophils. IFN-γ induces CCL13 in bronchial epithelial cell lines, endothelial cells, and chondrocytes, and a synergistic effect is obtained with INF-γ and TNF-α or IL-1 in chondrocytes. CCL13 is processed and inactivated by MMP-12.
Interaction: Monocytes, dendritic cells, basophils, eosinophils, T cells
Ligand/Receptor: CCR2, CCR3, and CCR5
Cell Type: Endothelial cells, Epithelial cells, Fibroblasts, Macrophages
Biology Area: Cell Biology, Signal Transduction
Molecular Family: Cytokines/Chemokines
Antigen References: Uguccioni M, et al. 1996. J. Exp. Med. 183:2379. Godiska R, et al. 1997. J. Leuko. Biol. 61:353. Kalayci O, et al. 2004. J. Asthma 41:27. Iwamoto T, et al. 2007. FEBS J. 274:4904. Mendez-Enriquez E, et al. 2008. Clin. Exp. Immunol. 152:354. Hintzen C, et al. 2009. Arthritis Rheum. 60:1932. Dean RA, et al. 2008. Blood 112:3455. Breland UM, et al. 2010. Cardiovasc. Res. 86:265.
Gene ID: 6357
UniProt: View information about CCL13 on UniProt.org
Clone: 3G4
Regulatory Status: RUO
Other Names: Monocyte chemeotactic protein 4 (MCP4), CKΒ10, New CC chemokine 1 (NCC1), Small inducible cytokine subfamily a, member 13 (SCYA13)
Isotype: Mouse IgG1, κ