Biolegend, 538304, Biotin anti-human Uncarboxylated Osteocalcin Antibody, 100μg

Osteocalcin is the most abundant non-collagenous protein found in the bone. While fully-carboxylated osteocalcin has a high affinity for the extracellular matrix of the bone; decreases in pH, caused by the process of bone resorption, result in decarboxylation of this protein. This generates both partially decarboxylated (undercarboxylated) and fully decarboxylated (uncarboxylated) osteocalcin molecules which are released into the circulation due to decreased affinity for the extracellular matrix. Fully carboxylated osteocalcin requires high calcium concentrations for proper protein folding; however, under/uncarboxylated osteocalcin has no calcium requirement to maintain its structure within the blood.
100μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Recombinant Human Decarboxylated (3xGLU) Osteocalcin
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography and conjugated with biotin under optimal conditions.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application: ELISA Detection - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by ELISA assay. For ELISA Detection applications, a concentration range of 1.0 - 4.0 µg/mL is recommended. To obtain a linear standard curve, serial dilutions of Human Uncarboxylated Osteocalcin recombinant protein (Cat. No. 446709) ranging from 37.5 to 2400 pg/mL is recommended for each ELISA plate. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: The biotinylated 4B6 antibody (Cat. No. 538304) is useful as the detection antibody in a sandwich ELISA assay, when used in conjunction with the pure 8H4 antibody (Cat. No. 538202) as the capture antibody and Human Uncarboxylated Osteocalcin synthetic peptide as the ELISA Std. (Cat. No. 446709).
RRID: AB_2888711 (BioLegend Cat. No. 538304)
Structure: Monomer
Distribution: It is produced by osteoblasts as a 49 amino acid protein, which contains 3 glutamine acid residues (Glu 17, 21, and 24) that are gamma carboxylated in a vitamin K dependent manner.
Function: Under/uncarboxylated forms act as hormones promoting β-cell proliferation, adiponectin secretion, insulin sensitivity, glucose tolerance, and testosterone biosynthesis. In patients with diabetes, reduced serum levels of osteocalcin are negatively correlated with obesity and insulin resistance. Furthermore, supporting studies in mice have suggested potential therapeutic applications for osteocalcin in both obesity and insulin resistance.
Interaction: GPRC6A, insulin, pancreatic β-cells, Ley-dig cells, adiponectin
Ligand/Receptor: Under/uncarboxylated forms can bind the receptor GPCR6a, calcium, and metal-binding.
Cell Type: Osteoblasts
Molecular Family: Hormones
Antigen References: Booth SL, et al. 2013. Nat Rev Endocrinol. 9:43. Diaz-Franco MC, et al. 2019. Mol Med Rep. 19:15-22. Karsenty G, et al. 2014. Mol Cell Endocrinol. 382:521. Zoch ML, et al. 2016. Bone. 82:42-9.
Gene ID: 632
UniProt: View information about Uncarboxylated Osteocalcin on UniProt.org
Clone: 4B6
Regulatory Status: RUO
Other Names: Osteocalcin, uncarboxylated osteocalcin, decarboxylated osteocalcin, 3xGlu, bone gamma-carboxyglutamate protein, Bone Gla Protein, BGP, OC, OCN, unOCN
Isotype: Mouse IgG1, κ
Q: How many biotin molecules are per antibody structure?
A: We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.