Biolegend, 570214, Cell-Vive™ GMP Recombinant Human IFN-γ (carrier-free), 25μg

Interferon-γ is a potent multifunctional cytokine which is secreted primarily by activated NK cells and T cells. Originally characterized based on anti-viral activities, IFN-γ also exerts anti-proliferative, immunoregulatory, and proinflammatory activities. IFN-γ plays a role in class I antigen presentation pathway by inducing catalytic proteasome subunit replacement with immunoproteasome subunit.  IFN-γ can upregulate MHC class I and II antigen expression by antigen-presenting cells. BioLegend Cell-Vive™ GMP Recombinant proteins are manufactured and tested in accordance with USP Chapter 1043, Ancillary Materials for Cell, Gene and Tissue-Engineered Products and Ph. Eur. Chapter 5.2.12 in a dedicated GMP facility compliant with ISO 13485:2016. Specifications and processes include:
25μg
Source: Human IFN-γ, amino acids Gln24-Gln166 (Accession # NM_000619) was expressed in E. coli.
Molecular Mass: The 144 amino acid N-terminal methionylated recombinant protein has a predicted molecular mass of 16,907 Da. The DTT-reduced protein and the non-reduced protein migrate at approximately 17 kDa by SDS-PAGE.
N-terminalSequence Analysis: Met-Gln-Asp-Pro-Tyr-Val-Lys-Glu-Ala-Glu
Purity: ≥ 95%, as determined by Coomassie stained SDS-PAGE
Formulation: 0.1 µm filtered protein solution is in 10 mM Sodium succinate, 5% Mannitol, pH 5.
Endotoxin Level: Less than or equal to 0.1 EU per μg protein as determined by the LAL method
Residual Host Cell Protein Content: ≤ 1 ng/μg by ELISA
Concentration: 500 µg/mL
Storage & Handling: Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% endotoxin-free BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week or stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
Activity: ED50 = 1 - 6 ng/mL as determined by the dose-dependent cytotoxicity of human colon cancer cell line HT-29 cells. Deep Blue Cell Viability™ Kit (Cat. No. 424701) is used to measure the proliferation.
Application: Bioassay Cell Culture
Application Notes: BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are verified in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at [email protected].
Product Citations: Wang Q, et al. 2024. Cell Rep Med. 101357:5. PubMed Lee SJ, et al. 2024. Heliyon. e26800:10. PubMed Lee SJ, et al. 2024. Sci Rep. 22601:14. PubMed
Disclaimer: BioLegend Cell-Vive™ GMP Recombinant proteins are for research use only. Suitable for ex vivo cell processing. Not for injection or diagnostic or therapeutic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.
Structure: Homodimer
Distribution: CD8+ and CD4+ T cells, NK cells
Function: Type II interferon produced by immune cells such as T-cells and NK cells that plays crucial roles in antimicrobial, antiviral, and antitumor responses by activating effector immune cells and enhancing antigen presentation. Upregulated by IL-2, FGF-basic, EGF; downregulated by vitamin D3 or DMN; labile at pH2.
Interaction: T cells, B cells, macrophages, NK cells, endothelial cells, fibroblasts
Ligand/Receptor: IFN-γRα (CDw119) dimerized with IFN-γRβ (AF-1)
Bioactivity: Measured by its ability to induce cytotoxicity in human colon cancer cell line HT-29 cells
Cell Type: Embryonic Stem Cells
Biology Area: Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience, Stem Cells
Molecular Family: Cytokines/Chemokines
Antigen References: Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press San Diego. De Maeyer E, et al. 1992. Curr. Opin. Immunol. 4:321. Farrar M, et al. 1993. Annu. Rev. Immunol. 11:571. Gray P, et al. 1987. Lymphokines 13:151.
Gene ID: 3458
UniProt: View information about IFN-gamma on UniProt.org
Other Names: Interferon-γ, Immune interferon, Type II interferon, T cell interferon, Macrophage-activating factor (MAF), IFNG, interferon gamma