Biolegend, 570804, Recombinant Human IL-6 (carrier-free), 25μg

IL-6 is a potent lymphoid cell growth factor that stimulates the growth and survival of certain B cells and T cells. IL-6 plays a role in host defense, acute phase reactions, immune response, and hematopoiesis. IL-6 is expressed by T cells, B cells, monocytes, fibroblasts, hepatocytes, endothelial cells and keratinocytes.
25μg
Source: Human IL-6, amino acids Pro29-Met212 (Accession # NM_000600) was expressed in E. coli.
Molecular Mass: The 185 amino acid N-terminal methionylated recombinant protein has a predicted molecular mass of 21,041 Da. The DTT-reduced and non-reduced protein migrate at approximately 23kDa by SDS-PAGE.
Purity: Purity is >98%, as determined by Coomassie stained SDS-PAGE.
Formulation: PBS, pH 7.2, 0.5M NaCl, 10% Glycerol
Endotoxin Level: Endotoxin level is <0.1 EU/µg (<0.01ng/µg) protein as determined by the LAL method.
Concentration: 10 and 25 µg sizes are bottled at 200 µg/mL. 100 µg size and larger sizes are lot-specific and bottled at the concentration indicated on the vial. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling: Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
Activity: The ED50 is 4-20 pg/ml, corresponding to a specific activity of 0.5-2.5 X108 units/mg, as determined by a dose dependent stimulation in a 7TD1 cell proliferation assay.The specific activity of recombinant human IL-6 is approximately 5.0 x 104 IU/µg when compared against the 1st WHO International Standard for Interleukin-6 (NIBSC code: 89/548) as determined by the dose dependent stimulation of 7TD1 cell proliferation.For more information on specific activity, please visit the Recombinant Protein Unit Conversions page.
Application: Bioassay
Recommended Usage: Use when high specific biological activity is required.
Application Notes: BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue-ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are verified in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at [email protected].
Additional Product Notes: View more applications data for this product in our Scientific Poster Library.
Application References(PubMed link indicates BioLegend citation): Reitsma JM, et al. 2013. J Virol. 87:10763. PubMed
Product Citations: Park SA, et al. 2023. Int J Mol Sci. 24:. PubMed Woodley K, et al. 2023. Nat Commun. 14:2132. PubMed Devi S, et al. 2023. Cell Rep. 112265:42. PubMed Khanam A, et al. 2022. Hepatology. 75:690. PubMed Sugawara E, et al. 2020. Autophagy. 1.323611111. PubMed Kaiser R, et al. 2021. JCI Insight. 6:e150862. PubMed Reitsma J, et al. 2013. J Virol. 87:10763. PubMed Zhang H, et al. 2020. Front Cell Dev Biol. 8:205. PubMed Baleeiro RB, et al. 2022. Oncoimmunology. 11:2080329. PubMed Guo J, et al. 2019. Cancer Immunol Res. 1.349305556. PubMed Gopinath A, et al. 2021. NPJ Parkinsons Dis. 7:62. PubMed Fulciniti M, et al. 2018. Cell Rep. 25:3693. PubMed Yamaguchi Y, et al. 2022. J Immunother Cancer. 10:. PubMed Ibrahim ML, et al. 2018. Cell Rep. 25:3036. PubMed Minns D, et al. 2021. Nat Commun. 12:1285. PubMed Miyamoto T, et al. 2021. Cancer Immunol Res. Online ahead of print. PubMed Guo Q, et al. 2022. Blood Adv. 6:5668. PubMed Wong GK, et al. 2019. JCI Insight. 4. PubMed Ianniciello A, et al. 2021. Sci Transl Med. 13:eabd5016. PubMed Jokela TA, et al. 2018. Front Cell Dev Biol. 6:41. PubMed Yang F, et al. 2021. Nat Commun. 12:3424. PubMed
Structure: Neuropoietic cytokine family
Distribution: T cells, B cells, macrophages, bone marrow stromal cells, fibroblasts, keratinocytes, mesangium, astrocytes, endothelial cells
Function: Upregulated by IL-1, TNF, PDGF, IFN-β, TNF-α, NGF, IL-17; downregulated by glucocorticoids IL-4, TGF-β
Interaction: T cells, B cells, hepatocytes, cholinergic neurons
Ligand/Receptor: Heterodimer IL-6Rα (CD126)/IL-6Rβ (CD130, gp130)
Bioactivity: Hematopoiesis; antigen-specific immune responses, inflammatory reactions; acute phase reaction
Cell Type: Hematopoietic stem and progenitors
Biology Area: Cell Biology, Immunology, Innate Immunity, Stem Cells
Molecular Family: Cytokines/Chemokines
Antigen References: 1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press San Diego. 2. Hirano T. 1998. Intl. Rev. Immunol. 16:249. 3. Patterson P. 1992. Curr. Opin. Neurobiol. 2:94. 4. Van Oers M, et al. 1993. Ann. Hematology 66:219.
Gene ID: 3569
UniProt: View information about IL-6 on UniProt.org
Regulatory Status: RUO
Other Names: Interleukin-6, B cell stimulating factor-2 (BSF-2), Cytotoxic T cell differentiation factor (CDF), Hepatocyte stimulating factor (HSF), Hybridoma/plasmacytoma growth factor (HPGF)
GMP Version Available: View Product
Q: Why choose BioLegend recombinant proteins?
A: • Each lot of product is quality-tested for bioactivity as indicated on the data sheet.
     • Greater than 95% Purity or higher, tested on every lot of product.
     • 100% Satisfaction Guarantee for quality performance, stability, and consistency.
     • Ready-to-use liquid format saves time and reduces challenges associated with reconstitution.
     • Bulk and customization available. Contact us.
     • Learn more about our Recombinant Proteins.
Q: How does the activity of your recombinant proteins compare to competitors?
A: We quality control each and every lot of recombinant protein. Not only do we check its bioactivity, but we also compare it against other commercially available recombinant proteins. We make sure each recombinant protein’s activity is at least as good as or better than the competition’s. In order to provide you with the best possible product, we ensure that our testing process is rigorous and thorough. If you’re curious and eager to make the switch to BioLegend recombinants, contact your sales representative today!
Q: What is the specific activity or ED50 of my recombinant protein?
A: The specific activity range of the protein is indicated on the product datasheets. Because the exact activity values on a per unit basis can largely fluctuate depending on a number of factors, including the nature of the assay, cell density, age of cells/passage number, culture media used, and end user technique, the specific activity is best defined as a range and we guarantee the specific activity of all our lots will be within the range indicated on the datasheet. Please note this only applies to recombinants labeled for use in bioassays. ELISA standard recombinant proteins are not recommended for bioassay usage as they are not tested for these applications.
Q: Have your recombinants been tested for stability?
A: Our testing shows that the recombinant proteins are able to withstand room temperature for a week without losing activity. In addition the recombinant proteins were also found to withstand four cycles of freeze and thaw without losing activity.
Q: Does specific activity of a recombinant protein vary between lots?
A: Specific activity will vary for each lot and for the type of experiment that is done to validate it, but all passed lots will have activity within the established ED50 range for the product and we guarantee that our products will have lot-to-lot consistency. Please conduct an experiment-specific validation to find the optimal ED50 for your system.
Q: How do you convert activity as an ED50 in ng/ml to a specific activity in Units/mg?
A: Use formula Specific activity (Units/mg) = 10^6/ ED50 (ng/mL)