Biolegend, 570809, Recombinant Human IL-6 (ELISA Std.), 4pack

IL-6 is a potent lymphoid cell growth factor that stimulates the growth and survival of certain B cells and T cells. IL-6 plays a role in host defense, acute phase reactions, immune response, and hematopoiesis. IL-6 is expressed by T cells, B cells, monocytes, fibroblasts, hepatocytes, endothelial cells and keratinocytes. Recombinant human IL-6 is a 26 kD protein containing 184 amino acids.
4pack
Source: The mature form of human IL-6, amino acids Pro 29-Met 212 (Accession # NM_000600) was expressed in E. coli.
Molecular Mass: The 185 amino acid N-terminal methionylated recombinant protein has a predicted molcular mass of 21,041 Da. The DTT-reduced and the non-reduced protein migrates at approximately 23kDa by SDS-PAGE.
Purity: Purity is >98% as determined by SDS PAGE , Coomassie stained with Invitrogen SimplyBlue SafeStain.
Formulation: Lyophilized in sterile-filtered PBS, pH 7.2, containing 1% BSA, 0.09% sodium azide, and protease inhibitors.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: Unopened vials can be stored between 2°C and 8°C until the expiration date. Prior to use, reconstitute the lyophilized powder with 0.2 mL of PBS containing a carrier protein (e.g., 1% BSA, protease free), pH7.4. Re-cap vial, vortex. Allow the reconstituted standard to sit at room temperature for 15 minutes, vortex again to mix completely. The reconstituted standard stock solution can be aliquoted into polypropylene vials and stored at -70°C for up to one month. Do not re-use diluted standards. Avoid repeated freeze/thaw cycles.
Application: ELISA
Recommended Usage: Each lot of this protein is quality control tested by ELISA assay. For use as an ELISA standard, a standard curve comprised of doubling dilutions from 1000 pg/ml to 8 pg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: This IL-6 protein is useful as a standard for a human IL-6 sandwich ELISA, using unlabeled MQ2-13A5 antibody (catalog #501102) for capture and biotinylated MQ2-39C3 antibody (catalog #501202) for detection.
Structure: Neuropoietic cytokine family
Distribution: T cells, B cells, macrophages, bone marrow stromal cells, fibroblasts, keratinocytes, mesangium, astrocytes, endothelial cells
Function: Upregulated by IL-1, TNF, PDGF, IFN-β, TNF-α, NGF, IL-17; downregulated by glucocorticoids IL-4, TGF-β
Interaction: T cells, B cells, hepatocytes, cholinergic neurons
Ligand/Receptor: Heterodimer IL-6Rα (CD126)/IL-6Rβ (CD130, gp130)
Bioactivity: Hematopoiesis; antigen-specific immune responses, inflammatory reactions; acute phase reaction
Biology Area: Immunology, Innate Immunity
Molecular Family: Cytokines/Chemokines
Antigen References: 1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press San Diego. 2. Hirano T. 1998. Intl. Rev. Immunol. 16:249. 3. Patterson P. 1992. Curr. Opin. Neurobiol. 2:94. 4. Van Oers M, et al. 1993. Ann. Hematology 66:219.
Gene ID: 3569
UniProt: View information about IL-6 on UniProt.org
Regulatory Status: RUO
Other Names: Interleukin-6, B cell stimulating factor-2 (BSF-2), Cytotoxic T cell differentiation factor (CDF), Hepatocyte stimulating factor (HSF), Hybridoma/plasmacytoma growth factor (HPGF)