Biolegend, 574116, Cell-Vive™ GMP Recombinant Human IL-23 (carrier-free), 500μg

Interleukin 23 (IL-23) is a member of the IL-12 family of cytokines, and it is comprised of two subunits, p19 and p40. The p19/p40 heterodimer is stabilized by a disulfide bond. The p40 subunit is shared by IL-23 and IL-12 cytokines; p19 mRNA is expressed in endothelial cells and polarized T cells, while p40 is not expressed by these cells. Therefore, the availability of functional IL-23 is limited by the expression of p40 and not p19. IL-23 exerts its biological activities through the interaction with a heterodimeric receptor complex composed of IL-12RB1 and IL-23R. Therefore, IL-23 and IL-12 share a common chain IL-12RB1 that interacts with p40. The second chain, IL-23R, interacts with p19. IL-23 activates Janus kinase (JAK)/signal transducer and activator of transcription (STAT) signaling molecules. JAK2 is constitutively associated with the IL-23R chain, and binding of IL-23 to its receptor leads to phosphorylation of STAT1, STAT3, STAT4, and STAT5. IL-23 has been associated with autoimmune inflammatory diseases such as psoriasis, colitis, gastritis, and arthritis. BioLegend Cell-Vive™ GMP Recombinant proteins are manufactured and tested in accordance with USP Chapter 1043, Ancillary Materials for Cell, Gene and Tissue-Engineered Products and Ph. Eur. Chapter 5.2.12 in a dedicated GMP facility compliant with ISO 13485:2016. Specifications and processes include:
500μg
Source: Human IL-23, amino acid Ile23-Ser328/Arg20-Pro189 [Accession # P29460 (p40) -Linker - Q9NPF7 (p19)] was expressed in CHO cells. The carboxy terminus contains a GGS-6His tag.
Molecular Mass: The 502 amino acid recombinant protein has a predicted molecular mass of approximately 55.6 kD. The DTT-reduced and non-reduced protein migrates at approximately 65 kD and 60 kD by SDS-PAGE, respectively. The predicted N-terminal amino acid is Ile.
N-terminalSequence Analysis: Ile-Trp-Glu-Leu-Lys-Lys-Asp-Val-Try-Val
Purity: ≥ 95%, as determined by Coomassie stained SDS-PAGE
Formulation: Protein was lyophilized from 0.1 μm filtered solution containing PBS and 5% Trehalose
Endotoxin Level: Less than or equal to 0.1 EU per μg of protein as determined by LAL method.
Residual Host Cell Protein Content: ≤ 0.500 ng/μg by ELISA
Concentration: 50 µg and 500 µg sizes are lyophilized
Storage & Handling: Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C or colder until the expiration date. Reconstitute lyophilized protein in sterile PBS. Before reconstitution, make sure sterile PBS and product are at room temperature. Quickly spin the vial or gently tap down on the vial to make sure the lyophilized product is at the bottom of the vial before opening. Use aseptic techniques to add the required volume of reconstitution buffer to the vial, to obtain the recommended stock concentration of 250 μg/mL. Close the vial and leave at ambient temperature for 15-20 minutes. Then gently invert the vial several times or until all the lyophilized product dissolves. Leave the vial at room temperature for another 15 minutes. If small particulates are still observed after 15 minutes, incubate at room temperature for an additional 30 minutes and leave the vial at 2°C - 8°C overnight. Next day, invert the vial several times or gently pipette the solution up and down before use. If needed, transfer the reconstituted stock solution to a sterile container for additional dilution to no less than 100 μg/mL. Small working aliquots in polypropylene tubes can be made after reconstitution and store the vials at -20°C or lower. Avoid freeze/thaw cycles. Carrier protein such as 0.2 - 1% endotoxin-free BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to two weeks or stored at -20°C or colder for up to 3 months.
Activity: Cell-Vive™ GMP Recombinant Human IL-23 induces IL-17A production in mouse splenocytes in a dose-dependent manner. The ED50 for this effect is 0.2 – 1.2 ng/mL.
Application Notes: Our lyophilized proteins are validated in-house to maintain activity after shipping at ambient temperature and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at [email protected].
Disclaimer: BioLegend Cell-Vive™ GMP Recombinant proteins are for research use only. Suitable for ex vivo cell processing. Not for injection or diagnostic or therapeutic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.
Structure: Heterodimer
Distribution: IL-23 is highly expressed by activated dendritic cells, macrophages, and epidermal Langerhans cells.
Function: IL-23 is the key cytokine in Th17 cell differentiation, Th17 cells differentiated from naive T cells are maintained and expanded by IL-23. IL-23 production by DCs is regulated by prostaglandin E2.
Interaction: Memory T cells, inflammatory macrophages, NK cells, dendritic cells, monocytes, and keratinocytes
Ligand/Receptor: IL-23 binds to L-12RB1 and IL-23R
Bioactivity: Human IL-23 induces the production of IL-17A in mouse splenocytes.
Antigen References: Oppmann B, et al. 2000. Immunity. 13:715. Aggarwal S, et al. 2003. J. Biol. Chem. 278:1910. Piskin G, et al. 2006. J. Immunol. 176:1908. McGeachy MJ, et al. 2007. Nat. Immunol. 8:1390. Wilson NJ, et al. 2007. Nat. Immunol. 8:950. Bonifac B, et al. 2008. Immunol. Rev. 226:132. Chizzolini C, et al. 2008. Blood. 112:3696. Buonocore S, et al. 2010. Nature. 464:1371. Harden JL, et al. 2015. J Autoimmun. 64:66. Fotiadou C, et al. 2018. Psoriasis (Auckl). 8:1.
Gene ID: 359351561
UniProt: View information about IL-23 on UniProt.org
Other Names: p19, IL-23 Subunit Alpha, IL-23p19, IL-23-A. p40, Interleukin-12 Subunit Beta, Interleukin 12B, IL-12B, Interleukin 12 P40