Biolegend, 577326, Cell-Vive™ GMP Recombinant Human PDGF-BB (carrier-free), 500μg

PDGF-BB is a mitogen initially identified as simian sarcoma viral oncogene homolog. It belongs to the PDGF family which includes five members, PDGF-AA, PDGF-BB, PDGF-AB, PDGF-CC, and PDGF-DD. PDGF-AA, AB, and BB dimers are processed intracellularly and secreted as active dimers that readily activate PDGF receptors (PDGFRs). PDGF-CC and DD are secreted as full-length, latent dimers, and the proteolytic removal of a CUB domain is required for the growth factor domains of PDGF-CC or DD to activate the PDGF receptors. PDGF-BB exerts its biological functions through the activation of dimeric receptors made up of two structurally similar proteintyrosine kinase receptor subunits (αα-, αβ-, or ββ-PDGFR). PDGF has been characterized as a chemoattractant for vascular smooth muscle cells from both sheep and humans and also for canine tracheal myocytes. In ex vivo cutaneous remodeling studies it was revealed that PDGF-BB is a mitogen and chemoattractant for dermal fibroblast. Abnormalities of PDGFR/PDGF are thought to contribute to a number of human diseases including malignancy. Autocrine signaling as a consequence of PDGF-B overexpression is clearly implicated in the pathogenesis of dermatofibrosarcoma protruberans (DFSP). BioLegend Cell-Vive™ GMP Recombinant proteins are manufactured and tested in accordance with USP Chapter 1043, Ancillary Materials for Cell, Gene and Tissue-Engineered Products and Ph. Eur. Chapter 5.2.12 in a dedicated GMP facility compliant with ISO 13485:2016. Specifications and processes include:
500μg
Source: Human PDGF-BB amino acid (Ser82-Thr190) (Accession: # NM_033016), was expressed in E.coli.
Molecular Mass: The 109 amino acid recombinant protein has a predicted molecular mass of approximately 12.2 kD. The DTT-reduced and non-reduced protein migrates at approximately 13 and 37 kD by SDS-PAGE respectively. The predicted N-terminal amino acid is Ser.
N-terminalSequence Analysis: Ser-Leu-Gly-Ser-Leu-Thr-Ile-Ala-Glu-Pro
Purity: ≥ 95%, as determined by Coomassie stained SDS-PAGE
Formulation: Protein was lyophilized from 0.2 μm filtered solution contain 0.1% TFA, 10% Acetonitrile.
Endotoxin Level: Less than or equal to 0.1 EU per μg protein as determined by the LAL method
Residual Host Cell Protein Content: ≤ 0.500 ng/μg by ELISA
Concentration: 100 μg size is lyophilized
Storage & Handling: Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C or colder until the expiration date. Reconstitute lyophilized protein in sterile 4 mM HCL. Before reconstitution, make sure sterile 4 mM HCL and product are at room temperature. Quickly spin the vial or gently tap down on the vial to make sure the lyophilized product is at the bottom of the vial before opening. Use aseptic techniques to add the required volume of reconstitution buffer (sterile 4 mM HCL) to the vial, to obtain the recommended stock concentration 250 μg/mL. Close the vial and leave at ambient temperature for 15-20 minutes. Then gently invert the vial several times or until all of the lyophilized product dissolves. Leave the vial at room temperature for another 15 minutes. If small particulates are still observed after 15 minutes, incubate at room temperature for an additional 30 minutes, and leave the vial at 2°C - 8°C overnight. Next day, invert the vial several times or gently pipette the solution up and down before use. If needed, transfer the reconstituted stock solution to a sterile container for additional dilution to no less than 100 μg/mL. Small working aliquots in polypropylene tubes can be made after reconstitution and store the vials at -20°C or lower. Avoid freeze/ thaw cycles. Carrier protein such as 0.2 - 1% endotoxin-free BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to two weeks or stored at -20°C or colder for up to 3 months.
Activity: Cell-Vive™ GMP Recombinant Human PDGF-BB induces the proliferation of NIH/3T3 cells in a dose-dependent manner. The ED50 for this effect is 1 - 5 ng/mL. The specific activity of Cell-Vive™ GMP Recombinant Human PDGF-BB (carrier-free) is ≥ 2.2 x 106 IU/mg when compared against the WHO International Standard for Human PDGF-BB (NIBSC code: 94/728).
Application: Bioassay
Application Notes: Our liophilized proteins are validated in-house to maintain activity after shipping at ambient temperature and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at [email protected].
Disclaimer: BioLegend Cell-Vive™ GMP Recombinant proteins are for research use only. Suitable for ex vivo cell processing. Not for injection or diagnostic or therapeutic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.
Structure: Homodimer
Distribution: PDGF-BB is mainly expressed in vascular endothelial cells, megakaryocytes, and neurons.
Function: PDGF-BB regulates diverse cellular processes, including cell proliferation, transformation, migration, survival, and apoptosis of mesenchymal cells, in development and during pathogenesis.
Interaction: Mesenchymal-derived cells
Ligand/Receptor: αα-PRGFR, αβ-PDGFR, and ββ-PDGFR
Bioactivity: Recombinant human PDGF-BB induces the proliferation of NIH/3T3 cells in a dose-dependent manner.
Antigen References: Carlin SM, et al. 2003. Am J Physiol Lung Cell MoPhysiol. 284:L1020. Ustach CV, et al. 2005. Mol Cell Biol. 25:6279. Jones AV and Cross NC. 2004. Cell Mol Life Sci. 61:2912. Andrae J, et al. 2008. Genes Dev. 22:1276. Banerjee P, et al. 2011. J. Biol Chem. 23:33580
Gene ID: 5159
UniProt: View information about PDGF-BB on UniProt.org
Other Names: Simian sarcoma viral oncogene homolog, v-SIS, SSV, SIS, c-sis, PDGF2