Biolegend, 598612, AZDye 647 Recombinant Human CCL5, 10μg

CCL5, also known as RANTES, is a CC chemokine that directs the migration of leukocytes in immune and inflammatory responses. Increased CCL5 expression has been associated with a wide range of inflammatory disorders and pathologies. It has been shown that CCL5 is highly expressed by breast tumor cells at primary tumor sites and is strongly associated with the progression of breast cancer. CCL5 may contribute to tumor progression by recruiting monocytes into tumor sites, and CCL5 can also stimulate the release of MMP, thereby promoting tumor cell migration. Some studies also show that CCL5 has pro-angiogenesis effects in vivo and in vitro . CCL5 oligomerization and binding to GAGs can induce angiogenic effects through its receptor CCR1 and CCR5. CCL5 is expressed in the early stages of many viral infections. It has been shown that CCL5 is protective against many viruses including influenza, RSV, HCMV, HCV, and HIV. CCL5 can inhibit HIV infection by competing with the virus for CCR5 binding. CCL5 also has been implicated in the development of asthma, atherosclerosis, and fibrosis.
10μg
Source: Human CCL5, amino acids Ser24-Ser91 (Accession # P13501) with a C-terminal AZDye647, was expressed in E. coli.
Molecular Mass: The 68 amino acid recombinant protein (Ser24-Ser91) has a predicted molecular mass of 7.8 kDa.
Purity: > 95%, as determined by mass spectrometry
Formulation: Lyophilized from 0.2 µm filtered solution of protein in DI-water.
Endotoxin Level: Less than 0.1 EU per µg cytokine as determined by the LAL method.
Concentration: Lyophilized, 10 µg per vial
Storage & Handling: Lyophilized at -20°C to -80°C until expirationLyophilized at 4°C for 6 monthsLyophilized at Room Temperature 1 monthReconstituted at -20°C to -80°C for 12 monthsReconstitution: For maximum results, quick spin vial prior to opening. The protein can be aliquoted under aseptic conditions and stored at -20°C or colder. Stock solutions can be prepared at concentrations of 250 µg/mL up to 1 mg/mL in 0.22 µm filtered DI-water. Avoid repeated freeze/thaw cycles.
Activity: Measured by its ability to recruit β-arrestin 2 in HEK293T cells transfected with human CCR5. The ED50 for this effect was measured at 8 to 30 ng/mL.
Application: FC, Live cell imaging
Application Notes: Protein has been verified for bioactivity prior to conjugation
Structure: CCL5 is a 68 amino acids molecule with a predicted molecular weight of 7.8 kDa. PDB ID – 1B3A
Distribution: CCL5 is secreted by lymphocytes, macrophages, eosinophils, platelets, and fibroblasts. CCL5 is expressed by hepatocytes, sinusoidal endothelial cells and biliary epithelium during chronic hepatitis C
Function: CCL5 can promote IL-2 and IL-5 expression in human T cells, and the expression of IL-6 and IL-8 in fibroblasts from patients with RA. CCL5 modulates LPS-induced IL-6 and TNFα in peripheral blood monocytes; this modulation occurs through the induction of IL-10 by CCL5.
Interaction: CCL5 is a chemoattractant for memory and activated T cells, monocytes, dendritic cells, eosinophils, NK cells, mast cells, and basophils
Ligand/Receptor: CCL5 binds to CCR1, CCR3, and CCR5 receptors. CCL5 also binds to CCR9 but does not induce calcium flux, and therefore there is no chemotactic response.
Bioactivity: Measured by its ability to recruit β-arrestin 2 in HEK293T cells transfected with human CCR5. The ED50 for this effect was measured at 8 to 30 ng/mL.
Cell Type: Basophils, Dendritic cells, Eosinophils, Leukocytes, Lymphocytes, Macrophages, Mast cells, Monocytes, NK cells, NKT cells, T cells
Biology Area: Cell Biology, Immuno-Oncology, Immunology, Innate Immunity
Molecular Family: Cytokines/Chemokines, Innate Immune Signaling
Antigen References: Schall TJ, et al. 1990. Nature. 347:669-71. Cocchi F, et al. 1995. Science. 270:1811-5. Shahrara S, et al. 2006. J Immunol. 177:5077-87. Larrubia JR, et al. 2008. World J Gastroenterol. 14:7149-59. Gleissner CA, et al. 2008. Arterioscler Thromb Vasc Biol. 28:1920-7. Marques RE, et al. 2013. Expert Opin Ther Targets. 17:1439-60.
Gene ID: P13501
UniProt: View information about CCL5 on UniProt.org
Regulatory Status: RUO
Other Names: SIS-delta, T-cell specific protein p288, Regulated on Activation Normal T-expressed and Secreted (RANTES), Chemokine (C-C Motif) Ligand 5, TCP228, C-C Motif Chemokine Ligand 5, Chemokine