Biolegend, 600455, Alexa Fluor® 647 anti-JunB Antibody, 25μg

JunB is a member of the AP-1 family of transcription factors, which also includes c-Jun, c-Fos, Fra1, and JunD. JunB can form dimers with other Jun and Fos proteins, as well as members of the ATF transcription factor family, to form a functional AP-1 transcription factor complex. AP-1 complexes containing JunB can exert both activator and repressor function depending on the interacting partner and promoter sequence context. JunB displays both tumor suppressor and oncogenic activity, inducing genes involved in cell senescence and repressing genes involved in proliferation. JunB is required for T helper type 2 (Th2), T helper 17 (Th17) cell and regulatory T cell (Treg) differentiation. Multiple cancers display aberrant JunB expression and function. In some lymphomas, notably Hodgkin’s lymphoma, JunB expression is elevated and the protein functions as an oncogenic transcription factor. Conversely, loss of JunB is observed in chronic myeloid leukemia.
25μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Partial recombinant human JunB protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: ICC - Quality tested ICFC - Verified
Recommended Usage: Each lot of this antibody is quality control tested by immunocytochemistry. For immunocytochemistry, a concentration range of 5.0 - 10.0 μg/mL is recommended. Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses
Excitation Laser: Red Laser (633 nm)
Additional Product Notes: For use in immunocytochemistry (ICC), it is recommended to fix/permeabilize with either of the following: -Fixation buffer (Cat. No. 420801) with 100% ice-cold methanol For use in intracellular flow cytometry (ICFC), it is recommended to fix/permeabilize with True-Phos™ Perm Buffer (Cat. No. 425401).
RRID: AB_3720594 (BioLegend Cat. No. 600455) AB_3720594 (BioLegend Cat. No. 600456)
Structure: JunB is a 347 amino acid protein with a predicted molecular weight of 36 kD.
Distribution: Nucleus
Function: Transcription Factor
Biology Area: Cell Biology, Cell Cycle/DNA Replication, Cell Proliferation and Viability, Transcription Factors
Antigen References: Shaulian E and Karin M. 2002. Nat Cell Biol. 4:E131-6. Yang MY, et al. 2003. Blood. 101:3205-11. Carr T, et al. 2017. Nat Commun. 8:301. Katagiri T, et al. 2019. Mucosal Immunol. 12:1104-1117.
Gene ID: 3726
UniProt: View information about JunB on UniProt.org
Clone: W20103F
Regulatory Status: RUO
Other Names: Activator Protein 1, AP-1
Isotype: Rat IgG2a, κ