Biolegend, 600856, Alexa Fluor® 647 anti-FosB Antibody, 100μg

FosB is a member of the FOS family of proteins that dimerizes with proteins of the Jun family to form the AP-1 transcription factor complex. As part of AP-1, FosB plays a critical role in regulating cell differentiation, proliferation, and apoptosis. FosB is expressed in most tissues and has been particularly well-studied in the CNS. ΔFosB, a truncated splice variant of the FOSB gene, plays a critical role in the brain’s reward circuit plasticity, with significant impact on both natural and pathological behavior. FosB also functions as a tumor suppressor, and dysregulation of FosB activity or levels has been found in numerous cancers, including gastric, prostate and colorectal cancer.
100μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Full-length recombinant FosB protein
Formulation: µg size: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide test sizes: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration: µg sizes: 0.5 mg/mLtest sizes: lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: ICFC (test sizes) - Quality tested ICC (µg size) - Quality tested
Recommended Usage: Each lot of the test-sized antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining using the test sizes, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. Each lot of the µg-sized antibody is quality control tested by immunocytochemistry. For immunocytochemistry using the ug size, a concentration range of 1.25 - 5.0 μg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses
Excitation Laser: Red Laser (633 nm)
Application Notes: Methanol only and PFA + Methanol fix/perm methods were tested and produced inferior signal. ICFC: Cells were fixed and permeabilizes using True Phos Buffer system ICC: Cells were fixed with 4% PFA and permeabilized with Triton X-100
RRID: AB_2941600 (BioLegend Cat. No. 600853) AB_2941600 (BioLegend Cat. No. 600856) AB_2941600 (BioLegend Cat. No. 600854)
Structure: FosB is a 338 amino acid protein with a predicted molecular weight of 36kD.
Distribution: FosB is widely expressed in developing and adult tissues, including the brain, thyroid, bladder and adipose tissue.
Function: FosB heterodimerizes with proteins of the Jun family to form the transcription factor AP-1 to regulate cell proliferation, survival, and differentiation.
Interaction: Jun family proteins e.g. c-Jun, genes with the TPA response element (TGA G/C TCA) SWI/SNF chromatin remodeling complex, DPP4
Cell Type: Mature Neurons
Biology Area: Cell Biology, Cell Proliferation and Viability, Neuroscience, Neuroscience Cell Markers, Transcription Factors
Molecular Family: Tumor Suppressors
Antigen References: Nestler E. 2015. Eur J Pharmacol. 753:66-72. Milde-Langosch K. 2005. Eur J Cancer. 41:2449-61.
Gene ID: 2354
UniProt: View information about FosB on UniProt.org
Clone: W19011B
Regulatory Status: RUO
Other Names: G0/G1 switch regulatory protein 3 (G0S3), FosB Proto-Oncogene, AP-1 Transcription Factor Subunit, Oncogene Fos-B, FBJ Murine Osteosarcoma Viral Oncogene Homolog B
Isotype: Rat IgG2a, κ