Biolegend, 601253, LEAF™ Purified anti-human CD218a (IL-18Rα) Antibody, 100μg

IL-18 is essential for both innate and adaptive immunity. The IL-18 receptor is composed of an α and a β subunit that form a high affinity heterodimer for IL-18. IL-18R1, also known as IL-18Rα chain and CD218a, is a type I transmembrane protein. It is expressed on NK cells, neutrophils, endothelial cells, and subsets of T and B cells. The expression of IL-18R1 and IL-18RAcP (IL-18Rβ) on lymphocytes are upregulated upon IL-12 stimulation. IL-23 and IL-18 synergistically enhance IL-18R1 expression on NK-like T cells. Upon IL-18 engagement, the intracellular TIR domain of IL-18R1 and IL-18RAcP recruits adaptor proteins, MyD88, and in turn recruits IRAK and TRAF6, activating NF-κB and MAPK pathways, which upregulates production of various inflammatory cytokines. The interaction of IL-18 and IL-18 receptor has been reported to be implicated in promotion of Th1 cytokine production and atherogenesis. IL-37 also binds to IL-18R1, but recruits IL-1R8 (SIGIRR) to activate anti-inflammatory responses.
100μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Recombinant human CD218a (IL-18Rα)
Formulation: 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
Endotoxin Level: Less than 0.1 EU/μg of the protein (< 0.01 ng/µg of the protein) as determined by the LAL test
Preparation: The LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration: The antibody is bottled at the concentration indicated on the vial. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling: Upon receipt, store frozen at -20°C. Make small volume aliquots if needed and avoid repeated freeze-thaw cycles to prevent denaturing the antibody.
Application: Neut - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by neutralizing the production of human IFN-γ induced by recombinant human IL-18 at 8 ng/mL in KG-1 cells. ND50 range: 1-7 ng/mL. It is recommended that the reagent be titrated for optimal performance for each application.
RRID: AB_3097579 (BioLegend Cat. No. 601253) AB_3097579 (BioLegend Cat. No. 601254)
Structure: Disulfide-linked homodimer
Distribution: Expressed on NK cells and neutrophils, IL-12 activated T and B cells, endothelial cells, and smooth muscle cells; highly expressed on Hodgkin's disease cell lines
Function: IL-18Rα forms a receptor complex with IL-18Rβ and mediates IL-18 function
Interaction: Human IL-18Rα inhibits the induction of IFN-γ by human IL-18 in human KG-1 cells
Ligand/Receptor: IL-18
Cell Type: B cells, Endothelial cells, Neutrophils, NK cells, T cells
Biology Area: Adaptive Immunity, Immunology, Signal Transduction
Molecular Family: Cytokine/Chemokine Receptors
Antigen References: Torigoe K, et al. 1997. J Biol Chem. 272:25737-42. Born TL, et al. 1998. J Biol Chem. 273:29445-50. Airoldi I, et al. 2000. J Immunol. 165:6880-8. van de Wetering D, et al. 2008. Int Immunol. 21:145-53. Adachi O, et al. 1998. Immunity. 9:143-50. Tsutsumi N, et al. 2014. Nat Commun. 5:5340.
Gene ID: 8809
UniProt: View information about CD218a on UniProt.org
Clone: W19242A
Regulatory Status: RUO
Other Names: IL-18 receptor alpha chain, IL-18 receptor 1, IL-18 receptor related protein, CDw218a
Isotype: Rat IgG2a, κ
Q: Do you guarantee that your antibodies are totally pathogen free?
A: BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.
Q: Does BioLegend test each Ultra-LEAF™ antibody by functional assay?
A: No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.
Q: Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?
A: No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.
Q: Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?
A: We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.