Biolegend, 601653, Ultra-LEAF™ Purified anti-human Oncostatin M Antibody, 100μg

Human oncostatin M (OSM) was initially isolated from supernatant of U937 cells treated with PMA. It was identified by its property to inhibit the proliferation of A375 melanoma cells and other human tumor cells. Bioinformatic homology analysis reveals that OSM has a similar composition and structure to LIF, G-CSF, and IL-6. OSM and LIF are located on human chromosome 22, and additional evidence suggests that these genes were derived by duplication of a common ancestral gene. In fact, many of its biological functions are shared with LIF. OSM binds two types of OSM receptor (OSMR) complexes. The type I receptor complex is composed of gp130 (IL-6 signal transducer) and LIF receptor β (LIFRβ) subunits. The type II receptor is constituted by the gp130 receptor and the OSMR β-chain. OSM also shares the OSMRβ-chain with the IL-31 receptor complex. The OSMRβ subunit is expressed by fibroblast, endothelial, hepatic, lung, and hematopoietic cells. The proinflammatory properties of OSM have been reported in skin, adipose tissue, lung, heart, and liver tissues. OSM triggers acute phase protein synthesis in hepatocytes. OSM is a potent inhibitor of keratinocyte proliferation and decreases the expression of both early and late keratinocyte differentiation markers. OSM transcripts are elevated in skin from psoriasis and atopic dermatitis patients, as compared with healthy skin. OSM and its receptor complexes play a key role in cutaneous inflammatory responses. In addition, OSM expression is increased in colonic biopsies of patients with active inflammatory bowel disease.
100μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Recombinant human Oncostatin M
Formulation: 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
Endotoxin Level: Less than 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.
Preparation: The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration: The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application: Neut - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by the inhibition of proliferation induced by recombinant human Oncostatin M (Cat. No. 555202) at 2.2 ng/mL on TF-1 cells. ND50 range: 0.5 -2 μg/mL. It is recommended that the reagent be titrated for optimal performance for each application.
RRID: AB_2910474 (BioLegend Cat. No. 601653) AB_2910474 (BioLegend Cat. No. 601654)
Structure: Cytokine
Distribution: Activated monocytes, macrophages, T cells including skin-infiltrating T cells, and dendritic cells
Function: Proinflammatory mediator and keratinocyte activator, induces acute phase protein synthesis, hematopoiesis, and bone remodeling
Interaction: Keratinocytes, fibroblasts, endothelia, hepatic cells, lung, hematopoietic cells, epithelial cells, and smooth muscle cells
Ligand/Receptor: Receptor complex: type I and type II complexes are formed by oncostatin M receptor (OSMR), LIFR, and p130 (IL-6 signal transducer).
Cell Type: Embryonic Stem Cells, Hematopoietic stem and progenitors
Biology Area: Cell Proliferation and Viability, Immunology, Stem Cells
Molecular Family: Cytokines/Chemokines, Growth Factors
Antigen References: Zarling JM, et al. 1986. Proc Natl Acad Sci USA. 83:9739-43. Rose TM and Bruce AG. 1991 Proc Natl Acad Sci USA. 88: 8641-5. Mosley B, et al. 1996. J Biol Chem. 271:32635-43. Dillon SR, et al. 2004. Nat Immunol. 5:752-60. Boniface K, et al. 2007. J Immunol. 178:4615-22. Beigel F, et al. 2014. PLoS One. 9:e93498. Schnittker D, et al. 2013. Mediators Inflamm. 2013:317503.
Gene ID: 5008
UniProt: View information about Oncostatin M on UniProt.org
Clone: A15044H
Regulatory Status: RUO
Other Names: OSM
Isotype: Mouse IgG2a, κ
Q: Do you guarantee that your antibodies are totally pathogen free?
A: BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.
Q: Does BioLegend test each Ultra-LEAF™ antibody by functional assay?
A: No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.
Q: Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?
A: No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.
Q: Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?
A: We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.