Biolegend, 603251, Purified anti-PGK1 Antibody, 25μg

Phosphoglycerate kinase 1 (PGK1) is one of two enzymes, along with its isozyme PGK2, which catalyzes the conversion of 1,3-diphosphoglycerate to 3-phosphoglycerate, and transfers the phosphate group to ADP to produce ATP. This step is notable as the “break even” point of glycolysis where ATP expenditure matches ATP output. Additionally, PGK1 functions as a protein kinase, and is also involved in transcription initiation as a cofactor for DNA polymerase alpha. The gene encoding PGK1 is on the X chromosome, and mutations in PGK1 are associated with various cancers, and X-linked recessive variants of anemia, mental disorders, and myopathy.
25μg
Verified Reactivity: Human, Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Partial fragment of recombinant Human PGK1
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested IHC-P, ICC - Verified
Recommended Usage: Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.25 - 1 µg/mL. For immunohistochemistry, a concentration range of 1 - 10 µg/mL is suggested. For immunocytochemistry, a concentration range of 1.25 - 5 μg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
RRID: AB_2910481 (BioLegend Cat. No. 603251) AB_2910481 (BioLegend Cat. No. 603252)
Structure: PGK1 is a 417 amino acid protein with a predicted molecular weight of 44.6 kD.
Distribution: PGK1 is widely expressed in the cytoplasm of most tissues.
Function: Enzyme catalyzing the conversion of 1,3-diphosphoglycerate to 3-phosphoglycerate. Protein kinase.
Interaction: PDHK1
Biology Area: Cancer Biomarkers
Molecular Family: Enzymes and Regulators, Protein Kinases/Phosphatase
Antigen References: Fu Q and Yu Z. 2020. Life Sci. 256:117863.
Gene ID: 5230
UniProt: View information about PGK1 on UniProt.org
Clone: W18295A
Regulatory Status: RUO
Other Names: Phosphoglycerate Kinase 1 (PGK1), cell migration-inducing gene 10 protein, primer recognition protein 2 (PRP2), Epididymis Secretory Sperm Binding Protein Li 68p, EC 2.7.2.3
Isotype: Rat IgG2a, λ