Biolegend, 603651, Purified anti-YAP1 Antibody, 25μg

Yes-associated protein 1, or YAP1, is a transcriptional co-activator vital to cell proliferation and the regulation of apoptosis. YAP1 does not contain a DNA-binding domain and requires interaction with transcriptional factors, particularly TEA domain (TEAD) proteins, to regulate transcription. YAP1 is continuously shuttled between the nucleus, where it is primarily located when Hippo signaling is inactive, and the cytoplasm, where it is sequestered following phosphorylation of its various serine residues by the LATS1/2-MOB1 complex of the Hippo pathway or by kinases AKT, PRP4K, and CDK1. As a key effector of the Hippo signaling pathway along with its paralog TAZ, YAP1 is essential to the regulation of cell growth, stem cell maintenance, organ size, tissue homeostasis, regeneration, embryonic development, and the preservation of genome integrity following DNA damage. YAP1 also functions in the modulation of aerobic glycolysis and glutaminolysis pathways as well as amino acid metabolism. YAP1 can act as a tumor suppressor, usually through its interaction with p53 family proteins and promotion of apoptosis, but most commonly functions as an oncogene. Its overexpression and deregulation are characteristic of several cancers and it has been implicated in the initiation, proliferation, metastasis, metabolic reprogramming, immune escape, and therapy resistance of cancer cells. As such, YAP1 is a potential therapeutic target and prognostic biomarker.
25μg
Verified Reactivity: Human, Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Recombinant human YAP1
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested ICC, IHC-P, ICFC - Verified
Recommended Usage: Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.25 - 1.0 µg/mL. For immunocytochemistry, a concentration range of 1.25 - 5.0 μg/mL is recommended. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 5.0 - 10.0 µg/mL is suggested. For intracellular flow cytometric staining, the suggested use of this reagent is 0.03 - 0.5 µg/test. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: For ICC, we recommend 4% PFA fixation followed by permeabilization with ice-cold methanol or 0.5% Triton-X. Fixation and permeabilization with only methanol is not recommended.
RRID: AB_2910483 (BioLegend Cat. No. 603651) AB_2910483 (BioLegend Cat. No. 603652)
Structure: YAP1 is a 504 amino acid protein with a predicted molecular weight of 54 kD.
Distribution: Ubiquitous/ Primarily nuclear localization at low density and primarily cytoplasmic localization at high density
Function: Transcription
Interaction: TAZ, TEAD1/2/3/4, LATS1/2, YES, SRC, SMAD, p73, RUNX2, PAX3, ERBB4, CK1, ZO1/2
Biology Area: Cancer Biomarkers, DNA Repair/Replication, Stem Cells, Transcription Factors
Antigen References: Chen X, et al. 2020. Front Physiol. 11:389. Isfort I, et al. 2019. Sci Rep. 9:19704. Raj N and Bam R. 2019. Front. Cell Dev Biol. 7:159. Szulzewsky F, et al. 2021. Dev Biol. 475:205-221.
Gene ID: 10413
UniProt: View information about Yap1 on UniProt.org
Clone: W19260A
Regulatory Status: RUO
Other Names: Yes1 Associated Transcriptional Regulator, YAP65, Yes-Associated Protein YAP65 Homolog, Transcriptional Coactivator YAP1, Yes Associated Protein 1, COB1, YAP2, YAP, YKI
Isotype: Rat IgG2b, κ