Biolegend, 603852, Purified anti-DNM1L Antibody, 100μg

Dynamin-1-like protein (DNM1L) is a member of the dynamin superfamily of GTPases. Its best studied role is in the control of mitochondrial fission. DNM1L oligomerizes into filaments that form rings around the scission site to “pinch off” dividing mitochondria using GTP hydrolysis. Accordingly, DNM1L also has important functions in the mitochondrial dynamics of cell proliferation and apoptosis. DNM1L is also required for proper formation of the nervous system, and deficiencies in its functions have been linked to Alzheimer’s Disease.
100μg
Verified Reactivity: Human, Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Recombinant human DNM1L protein fragments
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested ICC - Verified
Recommended Usage: Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.25 - 1.0 µg/mL. For immunocytochemistry, a concentration range of 1.0 - 2.5 μg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: For Western Blot, higher molecular weight band (≈100 kDa) likely corresponds to SUMOylated DNM1L (e.g. Figueroa-Romer, et al. 2009). For ICC, we recommend fixation/permeabilization with 100% ice cold methanol alone. Alternatively, 4% paraformaldehyde fixation followed by permeabilization with ice cold methanol is possible. We do not recommend 4% paraformaldehyde fixation followed by permeabilization with 0.5% Triton-X, due to incorrect signal localization. Not recommended for use in IHC-P (human).
RRID: AB_2922636 (BioLegend Cat. No. 603851) AB_2922636 (BioLegend Cat. No. 603852)
Structure: DNM1L is a 736 amino acid protein with a predicted molecular weight of 81.8 kD.
Distribution: DNM1L is widely expressed in the cytoplasm, mitochondria, and peroxisomes of most tissues.
Function: DNM1L is a GTPase
Interaction: CDK1
Biology Area: Cell Motility/Cytoskeleton/Structure, Mitochondrial Function
Molecular Family: Cytoskeletal Proteins, GTPases
Antigen References: Oliver D and Reddy PH. 2019. Cells. 8:961. Reddy PH, et al. 2011. Brain Res Rev. 67:103-18.
Gene ID: 10059
UniProt: View information about DNM1L on UniProt.org
Clone: W20081E
Regulatory Status: RUO
Other Names: Dynamin-1-like protein (DNM1L), Dnm1p/Vps1p-like protein (DVLP), Dynamin family member proline-rich carboxyl-terminal domain less (DYMPLE), Dynamin-like protein 1 (DLP1), Dynamin-related protein 1 (DRP1)
Isotype: Rat IgG2b, κ