Biolegend, 604353, Ultra-LEAF™ Purified anti-human CCL21 (6CKine) Antibody, 100μg

CCL21 is a CC chemokine highly expressed in lymph nodes. CCL21 is only 32% identical to CCL19 at the amino acid level; nevertheless, these chemokines bind to the same receptor CCR7.  CCL21 has a unique extension of about 30 amino acids with two extra cysteine residues in its carboxyl terminus. CCL21 is expressed in synovial tissue in rhematoid arthritis (RA), and it has been suggested that CCL21, but not CCL19, induces human microvascular endothelial cell (HMVEC) migration and/or tube formation. In addition, it has been reported that sustained high concentrations of either soluble or immobilized CCL19 and CCL21 inhibit T cell proliferation and IL-2 secretion.
100μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Recombinant human CCL21 (6CKine)
Formulation: 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
Endotoxin Level: Less than 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.
Preparation: The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration: The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application: Neut - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by neutralizing the chemotaxis induced by recombinant human CCL21 (6CKine) (Cat. No. 582202) on mouse Baf3- hCCR7 transfectants. ND50 range: 0.06 - 0.6 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.
RRID: AB_2922638 (BioLegend Cat. No. 604353) AB_2922638 (BioLegend Cat. No. 604354)
Structure: Chemokine
Distribution: CCL21 is highly expressed in HEV of lymph nodes and Peyer's patches. Also, it is expressed in the T cell zones of lymph nodes, in Peyer's patches by reticular cells, and in spleen by stromal cells. CCL21 is expressed by endothelial cells in lymphatic venules.
Function: CCL21 chemoattracts naïve T cells and maturing dendritic cells (DCs) into T zones of secondary lymphoid organs. CCL21 and CCL19 chemoattract antigen-engaged B cells to the boundary of B and T zones to interact with T-helper cells. CCL19 and CCL21 inhibit apoptosis in DCs.
Interaction: Thymocytes, activated T cells, naïve CD4 and CD8, mature DCs, fibroblast-like synoviocytes of patients with rheumatoid arthritis (RA)
Ligand/Receptor: CCR7 (CD197)
Cell Type: B cells, Dendritic cells, Tregs
Biology Area: Angiogenesis, Cell Biology, Immunology
Molecular Family: Cytokines/Chemokines
Antigen References: Hedrick JA and Zlotnik A. 1997. J. Immunol. 159:1589. Reif K, et al. 2002. Nature 416:94. Ziegler E, et al. 2007. J. Immunol. 179:6485. Pickens SR, et al. 2012. Arthritis Rheum. 10.1002.
Gene ID: 6366
UniProt: View information about CCL21 on UniProt.org
Clone: A15161B
Regulatory Status: RUO
Other Names: 6CKine, Secondary lymphoid tissue chemokine, SLC, Exudos 2, Small inducible cytokine subfamily A, member SCYA21
Isotype: Mouse IgG1, κ
Q: Do you guarantee that your antibodies are totally pathogen free?
A: BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.
Q: Does BioLegend test each Ultra-LEAF™ antibody by functional assay?
A: No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.
Q: Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?
A: No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.
Q: Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?
A: We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.