Biolegend, 604652, Purified anti-GSK3β Antibody, 100μg

GSK3B is a serine-threonine kinase and a member of the glycogen synthase kinase subfamily. It is a negative regulator of the hormonal control of glucose, Wnt signaling and also transcriptional factors and microtubules. It has been associated with regulation of several cellular mechanisms such as glycogen synthesis, protein synthesis, cell proliferation, microtubules stabilization, cell migration, autophagy and apoptosis. GSK3B is activated by phosphorylation at tyrosine 216 and inactivated by phosphorylation at serine 9. Defects on GSK3B has been associated with Alzheimer’s and Parkinson’s diseases.
100μg
Verified Reactivity: Human, Rat, Mouse
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Synthetic peptide conjugated to KLH
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: ICC - Quality tested IHC-P - Verified
Recommended Usage: Each lot of this antibody is quality control tested by immunocytochemistry. For immunocytochemistry, a concentration range of 1.25 - 5.0 μg/mL is recommended. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 4.0 - 6.0 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: For ICC, we recommend 4% PFA fixation followed by permeabilization with ice-cold methanol, or fixation/permeabilization with ice cold methanol alone. We do not recommend 4% PFA fixation followed by permeabilization with 0.5% Triton-X. For IHC-P, we recommend antigen retrieval using citrate buffer, HIER, and overnight antibody incubation at 4°C.
RRID: AB_2922641 (BioLegend Cat. No. 604651) AB_2922641 (BioLegend Cat. No. 604652)
Structure: GSK3B is a 420 amino acid protein with a mass of 46.7 kD
Distribution: GSK3B is expressed in the cytoplasm of testis, prostate, ovary and at lower levels in lung, brain and kidney
Function: GSK3B is a kinase involved in the regulation of several signaling pathways involving transcriptional factors, hormonal control of glucose, microtubule rearrangement, cell migration and proliferation, apoptosis and autophagy.
Biology Area: Cell Biology, Cell Proliferation and Viability, Protein Synthesis
Molecular Family: Protein Kinases/Phosphatase
Antigen References: Wu H, et al. 2019. Autophagy. 15:1506-1522. Edderkaoui M, et al. 2018. Gastroenterology. 155:1985-1998. Ryu HY, et al. 2021. Exp Mol Med. 53:369-383. Myöhänen TT, et al. 2021. Basic Clin Pharmacol Toxicol. 129:287-296. Taylan E, et al. 2020. Gynecol Oncol. 159:277-284. Mancinelli R, et al. 2017. Oxid Med Cell Longev. 2017:4629495.
Gene ID: 2932
UniProt: View information about GSK3beta on UniProt.org
Clone: 9B1-5C3-5G8
Regulatory Status: RUO
Other Names: glycogen synthase kinase 3 beta, Serine/Threonine-Protein Kinase GSK3B, Glycogen Synthase Kinase-3 Beta
Isotype: Mouse IgG1, κ