Biolegend, 605352, Purified anti-SMC3 Antibody, 100μg

Structural maintenance of chromosomes protein 3 (SMC3) is a member of the SMC protein family and a component of the cohesin complex. Cohesin plays an important role in mitosis and meiosis by mediating sister chromatid cohesion, homologous recombination, and spindle pole formation. SMC3 activity is mediated by phosphorylation at a pair of conserved lysine residues, stabilizing the cohesion complex during mitosis. Mutations in SMC3 can result in Cornelia de Lange syndrome, characterized by developmental disabilities.
100μg
Verified Reactivity: Human, Mouse
Reported Reactivity: X. laevis
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Synthetic fragment of X. laevis SMC3, raised from mice immunized with the peptide sequence: EEVSLRRVIGAKKDQYFLDKK, corresponding to amino acids 94 -114 of Xenopus laevis SMC3.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.25 - 1.0 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: Product was tested for use in ICC and IHC-P applications but did not meet passing criteria.
RRID: AB_2924611 (BioLegend Cat. No. 605351) AB_2924611 (BioLegend Cat. No. 605352)
Structure: SMC3 is a 1217 amino acid protein with a predicted molecular weight of 141.5 kD.
Distribution: SMC3 is present in the nucleus of cells. During mitosis, it segregates exclusively at the centrosomes of chromosomes.
Interaction: SMC3 is a component of the cohesion complex consisting SMC1, SCC1, and SCC3
Biology Area: Cell Biology, Cell Cycle/DNA Replication
Antigen References: Beckouet F, et al. 2010. Mol Cell. 39:689-99. Boyle MI, et al. 2015. Clin Genet. 88:1-12. Gligoris TG, et al. 2014. Science. 346:963-7.
Gene ID: 9126
UniProt: View information about SMC3 on UniProt.org
Clone: DJ5.2.3
Regulatory Status: RUO
Other Names: Structural maintenance of chromosomes protein 3 (SMC3), Basement membrane-associated chondroitin proteoglycan (BAMACAN), Chondroitin sulfate proteoglycan 6 (CSPG6), Chromosome-associated polypeptide (HCAP)
Isotype: Mouse IgG1, κ