Biolegend, 606201, Purified anti-C/EBP β (3 isoforms C/EBP β, LAP, LIP) Antibody, 25μg

C/EBP β (also known as CCAAT/enhancer-binding protein beta, interleukin 6-dependent DNA-binding protein, and nuclear factor of interleukin 6 [NF-IL6]) is a member of the basic leucine zipper C/EBP transcription factor family. C/EBP has three isoforms that include C/EBPβ, LAP, and LIP (35 kD, 32 kD, and 20 kD, respectively). This protein is localized in the nucleus and functions as a transcription factor in immune, inflammatory, acute-phase, cytokine, collagen type I gene expression. It is upregulated by growth hormones and has been shown to interact with CEBP-α, CEBP-δ, CEBP-γ, CEBP-ε, and CEBP-ζ. The C/EBP β protein can be modified by phosphorylation and ubiquitination. The 1H7 monoclonal antibody has been reported to be useful for Western blotting, immunoprecipitation, and immunofluorescence of mouse and rat C/EBP β, LIP, and LAP isoforms. The 1H7 antibody does not recognize human C/EBP β, LIP or LAP.
25μg
Verified Reactivity: Mouse, Rat
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Recombinant LIP
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested ChIP, IP, ICC - Reported in the literature, not verified in house
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 5 µg antibody per 5 mL antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: Recognizes three mouse isoforms: C/EBP ß, LAP, LIP. Does not cross-react with human.
Application References(PubMed link indicates BioLegend citation): Su, et al. 2003. J. Biol. Chem. 278:51150. (ChIP) Blidner AG, et al. 2015. J Immunol. 194:3452. PubMed
Product Citations: Huang JQ, et al. 2021. Front Mol Biosci. 8:725275. PubMed Ebert SM, et al. 2020. J Biol Chem. 295:2787. PubMed Blidner A, et al. 2015. J Immunol. 194:3452. PubMed Sharma N, et al. 2021. Front Mol Biosci. 7:603168. PubMed
RRID: AB_315675 (BioLegend Cat. No. 606202)
Structure: C/EBP transcription factor family, basic leucine zipper, dimer. Isoforms C/EBPβ, LAP, LIP, approximately 35 kD, 32 kD, 20 kD, respectively
Distribution: Nuclear
Function: Transcription factor in immune, inflammatory, acute-phase, cytokine, collagen type I genes
Interaction: CEBP-α, CEBP-β, CEBP-δ, CEBP-γ, CEBP-ε, CEBP-ζ
Modification: Phosphorylation, Ubiquitination
Biology Area: Cell Biology, Transcription Factors
Molecular Family: Nuclear Markers
Antigen References: 1. Chang C, et al. 1990. Mol. Cell Biol. 10:6642. 2. Eaton E, et al. 2001. J. Cell Physiol. 189:91. 3. Piwien-Pilipuk G, et al. 2002. J. Biol. Chem. 277:44557. 4. Hattori T, et al. 2003. Oncogene. 22:1273.
Regulation: Growth hormone
Gene ID: 1260825301
UniProt: View information about C/EBP beta on UniProt.org
Clone: 1H7
Regulatory Status: RUO
Other Names: CCAAT/enhancer-binding protein beta, Alpha-1-acid glycoprotein/enhancer binding protein, Interleukin 6-dependent DNA-binding protein (IL-6DBP), Nuclear factor of interleukin 6 (NF-IL6), Liver-enriched transcriptional activator protein (LAP)
Isotype: Mouse IgG2a, κ