Biolegend, 606302, Purified anti-Histone H2B Antibody, 100μg

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. Histones play central roles in transcription regulation, DNA repair, DNA replication and chromosomal stability. There are many variants of histones H2A and H2B. Specific H2A and H2B variants may preferentially associate with each other resulting in different combinations of variants and leading to the increased combinatorial complexity of nucleosomes. When being catalyzed by a series of histone modifying enzymes, histones may undergo various post-translational modifications such as acetylation, methylation, phosphorylation, ubiquitylation and SUMOylation. The dysregulation of histone modifying enzymes will alter the histone post-modification patterns and cause diverse diseases including cancers.
100μg
Verified Reactivity: Human, Mouse, Drosophila
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Human H2B1A peptide (111-127 a.a.)
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested IHC-P - Verified
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.25 - 2.0 µg per ml (1:250-2000). For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 1.0 - 5.0 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: This clone is not recommended for ChIP (Chromatin Immunoprecipitation) assays (as determined by in-house testing). Reactivity to Drosophila was only verified with the purified format.
Product Citations: Hayashi M, et al. 2021. Viruses. 13:. PubMed
RRID: AB_2728464 (BioLegend Cat. No. 606301) AB_2728465 (BioLegend Cat. No. 606302)
Structure: 126 amino acids with a predicted molecular weight of approximately 14kD but observed at approximately 17kD as the highly positively charged Histone protein tends to migrate slower on SDS-PAGE gel.
Distribution: Eukaryotic cells and nucleus.
Function: Histone H2B is one of the building blocks of nucleosome. It plays a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability.
Interaction: Histone H2A
Biology Area: Cell Biology, Chromatin Remodeling/Epigenetics, DNA Repair/Replication
Antigen References: 1. Ma F, Zhang CY. 2016. Expert Rev. Mol. Diagn. 16:297. 2. Li J, et al. 2016. Biomed. Rep. 4:293. 3. Shaytan AK, et al. 2015. Curr. Opin. Struct. Biol. 32:48. 4. Wright DE, Kao CF. 2015. Epigenetics 10:122. 5. Kim J, et al. 2009. Cell. 137:459. 6. Cheung WL, et al. 2003. Cell. 113:507.
Gene ID: 3018
UniProt: View information about Histone on UniProt.org
Clone: W17025A
Regulatory Status: RUO
Other Names: Histone Cluster 1, H2bb, H2B Histone Family-Member F, H2BFF, Histone H2B.1, H2B/F
Isotype: Rat IgG2a, λ