Biolegend, 606762, Alexa Fluor® 647 anti-Avi-tag Antibody, 100μg

Avi-tag is a 15 amino acid sequence, and the minimal acceptor peptide of a larger protein domain that serves as a substrate for the biotin ligase BirA from E. coli . Although one of the major advantages of Avi-tag fusion proteins is a controlled biotinylation in this domain, Avi-tag is also a useful tool in cell and molecular biology.
100μg
Verified Reactivity: Epitope tag
Reported Reactivity: Species independent
Antibody Type: Monoclonal
Host Species: Rat
Formulation: µg size: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide test sizes: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration: µg sizes: 0.5 mg/mLtest sizes: lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: ICFC (test sizes) - Quality tested ICC (µg size) - Quality tested
Recommended Usage: Each lot of the test-sized antibody is quality control tested by intracellular flow cytometry using our Cyto-Fast™ Fix/Perm Buffer Set. For flow cytometric staining, the suggested use of this reagent is 5 µL per 106 cells in 100 µL volume. It is highly recommended that the reagent be titrated for optimal performance for each application. Each lot of the µg-sized antibody is quality control tested by immunocytochemistry. For immunocytochemistry using the ug size, a concentration range of 1.25 - 5.0 μg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses
Excitation Laser: Red Laser (633 nm)
Application Notes: This clone recognizes proteins tagged with Avi-tag sequence irrespective of its biotinylation status.
Additional Product Notes: ICFC: Cells were fixed and permeabilizes using Cyto -Fast™ Buffer system ICC: Cells were fixed with 4% PFA and permeabilized with Triton X-100
RRID: AB_3068042 (BioLegend Cat. No. 606759) AB_3068042 (BioLegend Cat. No. 606762) AB_3068042 (BioLegend Cat. No. 606760)
Structure: GLNDIFEAQKIEWHE. Avi-tag amino acid sequence
Distribution: Depends on the subcellular location of the tagged protein
Biology Area: Cell Biology, Protein Purification, Protein Trafficking and Clearance
Gene ID: NA
UniProt: View information about Avi-tag on UniProt.org
Clone: W19258A
Regulatory Status: RUO
Other Names: Avi-Tag, AviTag, Avi epitope Tag
Isotype: Rat IgG2b, κ