Biolegend, 607802, Purified anti-RUNX1 Antibody, 100μg

RUNX1 belongs to the runt domain family of transcription factors and regulates target gene expression through forming a heterodimeric DNA-binding complex with CBFB. RUNX1 was first identified as a RUNX1-ETO fusion protein in acute myeloid leukaemia (AML) and is frequently mutated in AML and myelodysplastic syndrome due to chromosomal translocation. RUNX1-deficient mice fail to generate hematopoietic stem cells. RUNX1 regulates CD4 gene transcription during multiple stages of T cell development and represses the CD4 gene in CD4-CD8- (double negative) T cells. RUNX1 is also required for the differentiation of CD8+, Th17, and regulatory T cells. Impaired differentiation of megakaryocytes and decreased circulating platelet count was observed in the absence of RUNX1. These findings revealed that RUNX1 acts as a tumor suppressor for myeloid leukemia and is crucial for the development and terminal differentiation of several blood cell lineages.
100μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Recombinant human RUNX1 (amino acids 191-453) expressed in E. coli.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1:500-1:1000 diluted (0.5 – 1 µg/mL). It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: In contrast to BioLegend anti-RUNX1 clone 1C5B16, which shows reactivity towards both human and mouse RUNX1, clone W16118A is specific for human RUNX1 and does not cross-react with mouse RUNX1 (please see supplemental data). Clone W16118A (rat host) does not bind RUNX1 as strongly as 1C5B16 (mouse host), but still performed comparably to a competitor’s antibody.This clone is not recommended for ChIP (Chromatin Immunoprecipitation) assays (as determined by in-house testing).
RRID: AB_2721748 (BioLegend Cat. No. 607801) AB_2721749 (BioLegend Cat. No. 607802)
Structure: 453 amino acids encode a protein with a predicted MW of 48.7 kD. Contains a Runt domain responsible for DNA binding.
Distribution: Nucleus
Function: Plays crucial roles in hematopoiesis, hair follicle development, neurogenesis, chondrogenesis, and leukemogenesis.
Interaction: Interacts with TLE1, ELF1, ELF2, ELF4, SPI1, KAT6A, KAT6B, YAP1, and CDK6. Forms a heterodimer with CBFB.
Biology Area: Cell Biology, Neuroscience, Neuroscience Cell Markers, Transcription Factors
Molecular Family: Nuclear Markers
Antigen References: 1. Goyama S, et al. 2013. J. Clin. Invest. 123:3876. 2. Wang J, et al. 2013. PLoS One. 18:e74255. 3. Taniuchi I, et al. 2012. EMBO J. 31:4098. 4. Neel BG, et al. 2012. Genes Dev. 26:1520. 5. Della Gatta G, et al. 2012. Nat. Med. 18:436. 6. Hoi CS, et al. 2010. Mol. Cell Biol. 30:2518.
Gene ID: 861
UniProt: View information about RUNX1 on UniProt.org
Clone: W16118A
Regulatory Status: RUO
Other Names: AML1, CBFA2, PEBP2aB, RUNX1
Isotype: Rat IgG2a, κ