Biolegend, 607901, Purified anti-GAPDH Antibody, 25μg

GAPDH is well known for its glycolytic function of converting D-glyceraldehyde-3-phosphate to 1,3-bisphosphoglycerate. GAPDH is a ubiquitously expressed and has a molecular mass of 36 kD. Though differentially expressed from tissue to tissue, GAPDH is frequently used as a loading control for assays involving mRNA and protein detection. In more recent studies, GAPDH has been shown to be involved in microtubule bundling, prostate cancer progression, programmed neuronal cell death, DNA replication, and DNA repair. Recent work has elucidated roles for GAPDH in apoptosis, gene expression and nuclear transport. GAPDH may also play a role in neurodegenerative pathologies such as Huntington and Alzheimer's diseases.
25μg
Verified Reactivity: Human, Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Recombinant protein (161-335 a.a.)
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality testedICC - Verified
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.0375-0.5 µg per ml (1:1000-1:16000 dilution). For immunocytochemistry, a concentration range of 0.25-1.0 μg/ml (1:500-1:2000 dilution) is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: This clone weakly reacts with rat GAPDH.
Product Citations: Xie Q, et al. 2023. iScience. 26:106395. PubMed Qin XY, et al. 2023. Cell Death Dis. 14:358. PubMed Featherby SJ, et al. 2025. Mol Med Rep. :31. PubMed Su T, et al. 2021. Metabolites. 11:. PubMed Nchioua R, et al. 2022. J Virol. 96:e0059422. PubMed Hayn M, et al. 2021. Cell Reports. 35(7):109126. PubMed Hirschenberger M, et al. 2021. STAR Protoc. 2:100781. PubMed Nchioua R, et al. 2020. MBio. 11:00. PubMed Srinivasachar Badarinarayan S, et al. 2020. Nucleic Acids Res. 48:10890. PubMed Prelli Bozzo C, et al. 2021. Nat Commun. 12:4584. PubMed Bosso M, et al. 2021. Cell Rep. 36:109735. PubMed Bosso M, et al. 2021. STAR Protoc. 2:100236. PubMed Bosso M, et al. 2020. PLoS Pathog. 16:e1008752. PubMed Simon Langer et al. 2019. eLife. 8 pii: e41930. PubMed Stanfield BA, et al. 2021. PLoS One. 16:e0245169. PubMed Nchioua R, et al. 2021. J Virol. 95:e0080821. PubMed Chamberlain N, et al. 2022. Int J Mol Sci. 23:. PubMed Chen HL, et al. 2021. Pharm Biol. 59:134. PubMed Viereckl MJ, et al. 2022. Biomolecules. 12:. PubMed Hopfensperger K, et al. 2020. MBio. 11:00. PubMed Huq S, et al. 2020. Mol Cancer Ther. 19:1797. PubMed Hayashi M, et al. 2021. Viruses. 13:. PubMed Zhao Z, et al. 2021. PLoS Pathog. 17:e1009728. PubMed
RRID: AB_2734502 (BioLegend Cat. No. 607901) AB_2734503 (BioLegend Cat. No. 607902)
Structure: Belongs to the glyceraldehyde-3-phosphate dehydrogenase family, predicted molecular weight 36 kD. The enzyme exists as a tetramer of identical chains.
Distribution: Ubiquitously expressed, locate in cytoplasm and perinuclear region.
Function: Belongs to the glyceraldehyde-3-phosphate dehydrogenase family, predicted molecular weight 36 kD. The enzyme exists as a tetramer of identical chains.
Interaction: Interacts with TPPP. Interacts with EIF1AD and WARS. Interacts with SUMO4, GLUT4, nPKC-iota and CAMK2.
Biology Area: Cell Biology, Western Blot Controls
Antigen References: Ercolani L, et al. 1988. J. Biol. Chem. 263:15335. Meyer-Siegler K, et al. 1991. Proc. Natl. Acad. Sci. USA. 88:8460. Hara MR, Snyder SH. 2006. Cell. Mol. Neurobiol. 26:527. Zheng L, et al. 2003. Cell. 114:255. Wang Q, et al. 2005. FASEB J. 19:869. Bae BI, et al. 2006. Proc. Natl. Acad. Sci. USA. 103:3405.
Gene ID: 5590
UniProt: View information about GAPDH on UniProt.org
Clone: W17079A
Regulatory Status: RUO
Other Names: glyceraldehyde-3-phosphate dehydrogenase
Isotype: Rat IgG2a, κ