Biolegend, 608052, Purified anti-SLC1A5 Antibody, 100μg

Solute carrier family 1 member 5 (SLC1A5 or ASCT2) belongs to the SLC1 transporter family, which is used for exchanging neutral amino acids, especially glutamine uptake. SLC1A5 on the cytosolic membrane transports glutamine inwardly to regulate cellular energy metabolism, biosynthesis, cell proliferation, and signal transduction. In hematopoietic stem cells, SLC1A5 is necessary for erythroid cell differentiation. During placenta development, SLC1A5 serves as the receptor of syncytin-1 to stimulate trophoblast syncytialization. SLC1A5 is highly expressed in multiple cancers by positively regulating c-Myc, mTOR, and MAPK-PI3K signaling pathways to support tumor cell chemoresistance and cell growth. In addition, an SLC1A5 isoform variant induced by HIF-2α is expressed in mitochondria, that enhances metabolism and promotes drug resistance.
100μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Recombinant fragment of human SLC1A5 (400-541)
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested IHC-P, ICC, ICFC, IP - Verified
Recommended Usage: Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.25 - 1.0 µg/mL. For immunohistochemistry, a concentration range of 1.0 - 10.0 µg/mL is suggested. For immunocytochemistry, a concentration range of 1.25 - 5.0 μg/mL is recommended. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. For immunoprecipitation, the suggested use of this reagent is 2.5 µg/test. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: This product does not cross react with mouse in western blotting.
RRID: AB_2927964 (BioLegend Cat. No. 608051) AB_2927964 (BioLegend Cat. No. 608052)
Structure: SLC1A5 is a 541 amino-acid protein with a predicted molecular weight of 56 kD.
Distribution: SLC1A5 is expressed in placenta, lung, skeletal muscle, intestine, kidney, testis, T-cells, brain, and adipose tissue and is localized in cell membrane and cytosol.
Function: SLC1A5 transports neutral amino acids coupled with sodium.
Interaction: Interacts with suppressyn and syncytin-1
Ligand/Receptor: Sodium ion and neutral amino acid
Cell Type: Erythrocytes, Glutamatergic Neurons
Biology Area: Apoptosis/Tumor Suppressors/Cell Death, Cancer Biomarkers, Cell Biology, Cell Proliferation and Viability, Mitochondrial Function, Stem Cells
Molecular Family: Soluble Receptors
Antigen References: Hassanein M, et al. 2013. Clin Cancer Res. 19:560–70. Canul-Tec J, et al. 2017. Nature. 544:446-51. Yoo HC, et al. 2020. Cell Metab. 31:267-83.
Gene ID: 6510
UniProt: View information about SLC1A5 on UniProt.org
Clone: W19125A
Regulatory Status: RUO
Other Names: Solute carrier family 1 member 5, Sodium-dependent neutral amino acid transporter type 2, Neutral amino acid transporter B(0), ATB(0), Baboon M7 virus receptor, M7V1, RD114/simian type D retrovirus receptor, RDR, RDRC, ASCT2
Isotype: Rat IgG2a, κ