Biolegend, 608453, Alexa Fluor® 647 anti-RBFOX3 (NeuN) Antibody, 25μg

RBFox3, commonly referred to as NeuN, is one of a family of 3 mammalian Fox homologues. Fox was discovered in C. elegans as a gene involved in sex determination, and the name RBFox is an acronym of "Feminizing locus on X". The Fox protein and its 3 mammalian homologues are all about 46 kD proteins, each of which includes a central highly conserved RRM type RNA recognition motif, which corresponds to a small ~70 amino acid structure consisting of 4 beta strands and two alpha-helices. RBFox3 is a protein which has a function in RNA splicing and is expressed heavily and specifically in neuronal nuclei. The antibody reveals strong nuclear and distal cytoplasmic staining for RBFox3/NeuN and the complete absence of staining of astrocytes and other kinds of non-neuronal cells. This RBFox3/NeuN antibody is therefore an excellent marker of neuronal cells.
25μg
Verified Reactivity: Human, Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Full length recombinant human RBFOX3, isoform 5
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: IHC-P - Quality tested IHC-F - Verified
Recommended Usage: Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry in paraffin-embedded tissues, a concentration range of 1.0 - 10.0 µg/mL is suggested. For immunohistochemistry on frozen tissue sections, a concentration range of 1.0 - 10.0 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses
Excitation Laser: Red Laser (633 nm)
Additional Product Notes: For immunohistochemistry in paraffin-embedded tissues (IHC-P), we recommend antigen retrieval with either Sodium Citrate (Cat. No. 928502) or Tris-EDTA (10 mM Tris, 1 mM EDTA, 0.05% Tween-20, pH 9.0).
RRID: AB_3106161 (BioLegend Cat. No. 608453) AB_3106161 (BioLegend Cat. No. 608454)
Structure: RBFox3 is a 312 amino acid protein with a predicted molecular weight of 34 kD
Distribution: Mature Neurons, Neural Stem Cells/ Cytoplasm and Nucleus
Function: Cell Biology, Neuroscience, Neuroscience Cell Markers, Stem Cells
Cell Type: Mature Neurons, Neural Stem Cells
Biology Area: Cell Biology, Neuroscience, Neuroscience Cell Markers, Stem Cells
Molecular Family: Nuclear Markers
Antigen References: Dhekne HS, et al. 2018. Elife. 7:e40202. Sun Y, et al. 2020. J Neuroinflammation. 17:72. Schwabenland M, et al. 2021. Immunity. 54:1594-1610.
Gene ID: 146713
UniProt: View information about RBFOX3 on UniProt.org
Clone: W18230A
Regulatory Status: RUO
Other Names: NeuN, RNA binding protein fox-1 homolog 3 (RBFox3), hexaribonucleotide binding protein 3, HRNBP3
Isotype: Rat IgG2a, κ