Biolegend, 608602, Purified anti-RPS6 Phospho (Ser235/Ser236) Antibody, 100μg

Ribosomal protein S6 (RPS6) is a key component of the small 40S ribosomal subunit and is the major substrate of protein kinases in eukaryotic ribosomes. In response to various cellular stimuli such as mitogenic stimulation, insulin, and increased nutrient availability, upstream kinases such as RSK and p70 kinases phosphorylate RPS6 at multiple serine sites. These modifications facilitate the recruitment of the  7-methylguanasine cap complex, thereby promoting the assembly of the translational pre-initiation complex and increased cellular protein synthesis capacity. RPS6 has been shown to be hyperphosphorylated in certain cancers, and phosphorylation is a critical determinant of pancreatic β–cell size and systemic glucose homeostasis function in diabeteic mouse models.
100μg
Verified Reactivity: Human, Mouse
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Synthetic peptide from human RPS6 phosphorylated at Serines 235 and 236
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested ICC, ICFC, IHC-P - Verified
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.1 - 1.0 µg per ml. For immunocytochemistry, a concentration range of 1.0 - 5.0 μg/ml is recommended. For intracellular flow cytometry using our True-Phos™ Perm Buffer in Cell Suspension, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µl volume. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration of 10.0 µg/mL is suggested.It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: Due to complete conservation of the immunizing sequence between humans, mouse and rat, this clone is is predicted to react with rat RPS6 phosphorylated at serines 235 and 236.
Product Citations: Moore AR, et al. 2022. Cell Rep. 41:111651. PubMed
RRID: AB_2749900 (BioLegend Cat. No. 608601) AB_2749899 (BioLegend Cat. No. 608602)
Structure: RPS6 is a 249 amino acid protein with a predicted molecular weight of 28.6 kD.
Distribution: Cytosol, nucleus, ER, ubiquitous expression.
Function: Phosphorylation events at a cluster of serine residues (Ser 235, 236, 240, and 244) at the carboxy-terminus of RPS6 results in increased global translational efficiency. These sites are dephosphorylated during growth arrest.
Interaction: RPS6AK1 and RPS6AK3; other subunits of 40S ribosomal complex.
Biology Area: Cell Biology, Cell Proliferation and Viability, Protein Synthesis, Signal Transduction
Molecular Family: Nuclear Markers, Phospho-Proteins
Antigen References: Jefferies HB, et al. 1997. EMBO J. 16:3693. Ruvinsky I, et al. 2005. Genes. Dev.19:2199. Chumacher AM, et al. 2006. Biochemistry. 45:13614 Roux PP, et al. 2007. J. Biol. Chem. 282:14056. Stevens C, et al. 2009. J. Biol. Chem. 284:334. Schlafli P, et al. 2011. FEBS J. 278:1757.
Gene ID: 6194
UniProt: View information about RPS6 Phospho Ser235/Ser236 on UniProt.org
Clone: A17020B
Regulatory Status: RUO
Other Names: S6, 40S ribosomal protein S6
Isotype: Mouse IgG1, κ