Biolegend, 608752, Purified anti-SDHB Antibody, 100μg

SDHB encodes one of the four proteins that form the SDH (succinate dehydrogenase) enzyme complex in the mitochondrial-respiratory chain, and plays an important role in the mitochondria. The SDH complex functions to couple the conversion of succinate to fumarate in the mitochondrial matrix as part of the Krebs cycle with the reduction of ubiquitin to ubiquinol as part of the ETC (electron transport chain). Within the complex itself SDHB provides an attachment site for released electrons to be transferred to the oxidative phosphorylation pathway. Sporadic and familial mutations in this gene result in paragangliomas, pheochromocytoma, and gastrointestinal stromal tumors.
100μg
Verified Reactivity: Human, Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Recombinant fragment of human SDHB
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: IHC-P - Quality tested ICC, WB - Verified
Recommended Usage: Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 1.0 - 10 µg/mL is suggested. For immunocytochemistry, a concentration range of 1.25 - 5 μg/mL is recommended. For western blotting, the suggested use of this reagent is 0.25 - 1.0 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: Clone W19244A is suitable for ICC using methanol only as the fixation/permeabilization method. Alternate methods such as fixation with PFA followed by permeabilization with either methanol or Triton-X-100 resulted in staining in the wrong localization.
RRID: AB_2927962 (BioLegend Cat. No. 608751) AB_2927962 (BioLegend Cat. No. 608752)
Structure: SDHB is a 280 amino-acid protein with a predicted molecular weight of 32 kD.
Distribution: SDHB is present in the mitochondria.
Function: SDHB provides an attachment site for electrons as they are transferred to the oxidative phosphorylation pathway.
Interaction: SDHB forms the SDH (succinate dehydrogenase) enzyme complex along with SDHA, SDHC, and SDHD.
Biology Area: Cell Biology, Mitochondrial Function, Ubiquitin/Protein Degradation
Molecular Family: Enzymes and Regulators, Mitochondrial Markers, Tumor Suppressors
Antigen References: Seedat F, et al. 2021. SDHB Gene Mutation Associated Paraganglioma Syndrome -A Review. 4:118. Gill AJ. 2012. Pathology. 44:285-92.
Gene ID: 6390
UniProt: View information about SDHB on UniProt.org
Clone: W19244A
Regulatory Status: RUO
Other Names: Iron-Sulfur Subunit of Complex II, Succinate Dehydrogenase [Ubiquinone] Iron-Sulfur Subunit, SDHIP, SDH2, PGL4, CWS2, Succinate Dehydrogenase Complex Subunit B
Isotype: Rat IgG2a, κ