Biolegend, 609102, Purified anti-IκB-α Antibody, 100μg

IκB-α is an inhibitory protein that binds to the NF-κB complex and retains this complex in the cytoplasm where it cannot activate transcription. Upon cellular stimulation, the IκB proteins are phosphorylated and degraded via the ubiquitin pathway allowing for nuclear translocation of NF-κB. The NF-κB complex can then bind DNA to activate gene expression of multiple proteins involved in inflammation, cellular proliferation, differentiation, and apoptosis.
100μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Mouse IkB-α recombinant protein (full length) expressed in E. coli.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested IP - Verified
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.125-1.0 µg per ml (dilution of 1:500-1:4000). For immunoprecipitation, the suggested use of this reagent is 2.5 - 3.00 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: Weakly cross reacts with human I?Ba. This clone can not be used for ICC application.
Product Citations: Baccianti F, et al. 2023. MBio. e0178423:14. PubMed
RRID: AB_2734508 (BioLegend Cat. No. 609101) AB_2734509 (BioLegend Cat. No. 609102)
Structure: 314 amino acids, with predicted molecular weight 35.1 kD. Inhibitory protein of the NF-κB complex that contains multiple ankyrin repeats.
Distribution: Cytoplasm
Function: Regulates transcriptional activity of NF-κB by binding the complex and trapping it in the cytoplasm. Serine phosphorylation of IκB targets it for destruction via the ubiquitination pathway, which allows the NF-κB complex to translocate to the nucleus.
Interaction: Interacts with NFκB1, SUMO4, p53, ribosomal S6 kinase 1.
Biology Area: Cell Biology, Signal Transduction
Antigen References: Baeuerle PA. 1998. Cell. 95:729. Hoffmann A, et al. 2002. Science. 298:1241. Ito CY, et al. 1995. Genomics. 29:490.
Gene ID: 18035
UniProt: View information about IkappaB-alpha on UniProt.org
Clone: W16166B
Regulatory Status: RUO
Other Names: Nuclear factor of kappa light chain enhancer in B cells-inhibitor alpha, IKBA, IκBα
Isotype: Rat IgG2a, κ