Biolegend, 609252, Purified anti-SHP-2 Antibody, 100μg

Src homology-2-containing protein tyrosine phosphatase 2 (SHP-2) protein, encoded by the PTPN11 gene, is a well-known oncogenic protein tyrosine phosphatase. SHP-2 regulates cellular functions by dephosphorylating targets downstream of PD1, EGFRvIII, HGF receptor, and cytokine receptors. Cellular processes controlled by SHP-2 include mitotic cycle, cell growth, differentiation, and oncogenesis. SHP-2 has been recognized as a potential therapeutic target for cancers including breast cancer, lung cancer, leukemia, gastric cancer, and oral cancer.
100μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Recombinant fragment of human SHP-2
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested IHC-P - Verified
Recommended Usage: Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.25 - 1.0 µg/mL. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 5.0 - 10.0 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: This product cross reacts with mouse cell lines in Western-blot application but has poor selectivity. Clone A20016A is recommended for mouse reactivity. This product is not recommended for immunocytochemistry.
RRID: AB_2927989 (BioLegend Cat. No. 609251) AB_2927989 (BioLegend Cat. No. 609252)
Structure: SHP-2 is a 593 amino acid protein with a molecular mass of 68 kD
Distribution: Cytoplasm and Nucleus
Function: SHP-2 regulates cell proliferation, apoptosis, invasion, and metastasis
Interaction: SHP-2 interacts with PD1, EGFR, HGF receptor, RET, FGFR2, FGFR3, PI3K/AKT, and TLR3 signaling pathways.
Cell Type: Lymphocytes, T cells, Th1
Biology Area: Cell Biology, Cell Proliferation and Viability, Immuno-Oncology, Immunology
Molecular Family: Protein Kinases/Phosphatase
Antigen References: Hao C, et al. 2020. Oncogene. 39:7166-7180. Marie D, et al. 2008. Cell Signal. 20:453-9. Cheng Kui QU. 2000. Cell Research. 10:279-288.
Gene ID: 5781
UniProt: View information about SHP-2 on UniProt.org
Clone: W18298A
Regulatory Status: RUO
Other Names: CFC, NS1, JMML, SHP2, BPTP3, PTP2C, METCDS, PTP-1D, SH-PTP2, SH-PTP3, Syp, Protein Tyrosine Phosphatase Non-Receptor Type 11, SH2 Domain-Containing Protein Tyrosine Phosphatase 2
Isotype: Rat IgG2a, λ