Biolegend, 609802, Purified anti-SSRP1 Antibody, 100μg

SSRP1 is a nuclear protein and a component of the FACT complex (facilitates chromatin transcription). The FACT140 complex is composed of the Spt16 and the SSRP1 proteins. This complex interacts with nucleosomes and histone H2A/H2B dimers to promote nucleosome disassembly and allow transcription elongation. This protein interacts with dsDNA. The SSRP1 protein has been shown to be modified by cisplatin and may protect DNA from repair and block DNA replication. The 3E4 monoclonal antibody recognizes human SSRP1 and has been shown to be useful for Western blotting.
100μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Recombinant (partial)
Formulation: This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide at 0.5 mg/ml.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: Upon receipt, store undiluted between 2°C and 8°C.
Application: WB - Quality testedICC - VerifiedIP - Reported in the literature, not verified in house
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 5 µg per 5 ml antibody dilution buffer for each mini-gel. For immunocytochemistry, a concentration range of 2.5 - 10 μg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: This clone is not recommended for ChIP (Chromatin Immunoprecipitation) assays (as determined by in-house testing).
Application References(PubMed link indicates BioLegend citation): Tan B C-M, et al. 2006. EMBO doi:10.1038/sj.emboj.7601271
Product Citations: Horiuchi S, et al. 2021. Sci Immunol. :eabm3131. PubMed
RRID: AB_2195751 (BioLegend Cat. No. 609802)
Structure: SSRP family, HMG box domain; 81 kD
Distribution: Nuclear
Function: Interacts with nucleosomes and histone H2A/H2B dimers to promote nucleosome disassembly and allow transcription elongation, binds to dsDNA. Modified by cisplatin, may protect DNA from repair and block DNA replication
Interaction: Component of FACT ATP-dependent chromatin remodeling complex, Spt16/Cdc68 in FACT complex, H2A/H2B dimers
Biology Area: Cell Biology, Chromatin Remodeling/Epigenetics, DNA Repair/Replication, Transcription Factors
Antigen References: 1. LeRoy G, et al. 1998. Science 282:1836. 2. Orphanides G, et al. 1998. Cell 92:105. 3. Orphanides G, et al. 1999. Nature 400:284.
Gene ID: 6749
UniProt: View information about SSRP1 on UniProt.org
Clone: 3E4
Regulatory Status: RUO
Other Names: Structure specific recognition protein 1 (SSRP1), Facilitates Chromatin Transcription 80 kD subunit, FACTp80, Recombination signal sequence recognition protein
Isotype: Mouse IgG1, κ