Biolegend, 610151, Purified anti-VEGFR2 Phospho (Tyr951) Antibody, 50microl

VEGFR2 is a tyrosine kinase receptor located in the cell surface. It serves as a receptor for VEGF-A, -E, -C, and -D. Interaction of VEGF and VEGFR2 induces dimerization of the receptor followed by transphosphorylation on its cytoplasmic domains. Phosphorylation sites include Tyr801, Tyr951, Tyr1054, Tyr1059, Tyr1175, Tyr1214, Tyr1223, Tyr1305, Tyr1309, and Tyr1319. Phosphorylation at the Y951 residue facilitates binding to the SH2 domain of T-cell-specific adaptor (TSAd), which leads to activation of c-Src tyrosine kinase and vascular permeability.
50microl
Verified Reactivity: Human
Antibody Type: Polyclonal
Host Species: Rabbit
Immunogen: Synthetic peptide corresponding to human VEGFR2 phosphorylated at Tyrosine 951
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested dilution of this reagent is 1:2000. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: This clone was tested for Western-Blot on lysates from Huvec cells stimulated with or without VEGF-165.
RRID: AB_2928029 (BioLegend Cat. No. 610151) AB_2928029 (BioLegend Cat. No. 610152)
Structure: VEGFR2 is a 1,356 amino acid protein with a predicted molecular weight of 151 kD.
Distribution: VEGFR2 is expressed in endothelial cells. It is also upregulated in some cancer cells.
Function: Activation of VEGFR2 plays an essential role in angiogenesis, vascularization, cell proliferation, cell survival and migration. Its activation can be modulated by VEGFR1 that can sequester VEGF avoiding its binding to VEGFR2.
Interaction: Interacts with SHC2, Annexin A5, SHC1
Ligand/Receptor: VEGFR2 serves as a receptor for VEGF-A, -E, -C, and -D
Cell Type: Endothelial cells
Biology Area: Angiogenesis, Cell Biology, Immunology
Molecular Family: CD Molecules, Phospho-Proteins
Antigen References: Matsumoto T, et al. 2005. EMBO J. 24:2342-53. Sun Z, et al. 2012. J Exp Med. 209:1363-77. Abhinand C, et al. 2016. J Cell Commun Signal. 10:347-354. Clegg L, et al. 2015. PloS Comput Biol. 11:e1004158.
Gene ID: 3791
UniProt: View information about VEGFR2 Phospho Tyr951 on UniProt.org
Clone: Poly21A10
Regulatory Status: RUO
Other Names: Vascular endothelial growth factor receptor 2, VEGF-receptor 2, Kinase insert domain receptor, KDR, CD309, Flk1
Isotype: Rabbit Polyclonal IgG