Biolegend, 610951, Purified anti-PDHA1 Antibody, 25μg

PDHA1, pyruvate dehydrogenase E1 subunit alpha 1, is a major mitochondrial component of pyruvate dehydrogenase complexes (PDCs) which catalyzes oxidative decarboxylation of pyruvate in glycolysis and produces acetyl-CoA for the tricarboxylic acid (TCA) cycle. PDHA1 and PDHB form the heteroterameric pyruvate dehydrogenase E1 (E1) subunit of the PDC. The enzymatic activity of the PDC is regulated by phosphorylation of PDHA1 through pyruvate dehydrogenase kinase (PDK) and pyruvate dehydrogenase phosphatases (PDP). PDCs play crucial physiological functions between fed and fasting states which regulate pyruvate metabolism to maintain glucose homeostasis. PDHA1 enzymatic dysfunction causes fatal lactic acidosis and chronic neurologic dysfunction in the central nervous system. The decreased expression of PDHA1 in Parkinson’s disease and dementia with Lewy bodies patients suggests a dysfunction of mitochondrial energy production. Additionally, dysregulation of PDHA1 is found in multiple cancers, including lung, ovarian, stomach, and kidney, suggesting a role for PDHA1 in tumorigenesis.
25μg
Verified Reactivity: Human, Mouse
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Recombinant fragment of human PDHA1
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested IHC-P, ICC - Verified
Recommended Usage: Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.25 - 1.0 µg/mL. For immunocytochemistry, a concentration range of 1.25 - 5.0 μg/mL is recommended. For immunohistochemistry, a concentration range of 1.0 - 10.0 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: The suggested ICC fixation/permeabilization method is 4% paraformaldehyde plus methanol or ice-cold methanol only. Permeabilization with Triton X-100 is not recommended. For immunohistochemistry, the suggested antigen retrieval buffer is Tris-EDTA pH 9.0.
RRID: AB_2936739 (BioLegend Cat. No. 610951) AB_2936739 (BioLegend Cat. No. 610952)
Structure: PDHA1 is a 390 amino-acid protein with a predicted molecular weight of 43.3 kD that forms a heterotetramer with PDHB.
Distribution: Ubiquitous/mitochondria
Function: A subunit of pyruvate dehydrogenase E1 catalyzing the oxidative decarboxylation of pyruvate with the formation of acetyl-CoA and CO2, as part of pyruvate dehydrogenase complex for TCA cycle
Interaction: Forms a heteroteramer with PDHB; interacts with other pyruvate dehydrogenase complex components, E2 and E3.
Biology Area: Mitochondrial Function, Neurodegeneration
Molecular Family: Enzymes and Regulators, Mitochondrial Markers, Organelle Markers
Antigen References: Zhang C, et al. 2011. Proc Natl Acad Sci U S A. 108:16259-64. Patel MS, et al. 2014. J Biol Chem. 289:16615-23. Sperl W, et al. 2014. J Inherit Metab Dis. 38:391-403. Miki Y, et al. 2017. Biochem Biophys Res Commun. 489:439-444. Deng L, et al. 2022. Front Pharmacol. 13:947372.
Gene ID: 5160
UniProt: View information about PDHA1 on UniProt.org
Clone: 3H2-F8-B5
Regulatory Status: RUO
Other Names: Pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial, Pyruvate Dehydrogenase E1 Subunit Alpha 1, Pyruvate Dehydrogenase (Lipoamide) Alpha 1, E1alpha
Isotype: Mouse IgG1, κ