Biolegend, 611004, PE anti-HDAC1 Phospho (Ser406) Antibody, 100tests

Histone Deacetylase 1 (HDAC1) plays a critical role in various cellular processes, including cell cycle progression, proliferation, and differentiation. The enzyme functions by removing acetyl moieties from histone targets, resulting in histone compaction and alterations in nucleosomal positioning.  Aurora kinases phosphorylate HDAC1 at Ser406 during prophase, immediately after cells begin mitosis, resulting in reduced deacetylase activity of HDAC1. This modification plays an essential role in regulating cell cycle progression, as well as controlling the expression of genes involved in central nervous system development.
100tests
Verified Reactivity: Human, Zebrafish
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Synthetic peptide corresponding to human HDAC1 phosphorylated at Serine 406
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: ICFC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by intracellular flow cytometry using our True-Phos™ Perm Buffer in Cell Suspensions Protocol. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: When tested for western blot, this clone produced a band that showed a ~1-3 kD mass shift compared to a pan HDAC1 antibody. This observation is consistent with a previous study of the HDAC1 Phospho (Ser406) site. This clone recognizes zebrafish HDAC1 phosphorylated at Ser4062. Clone BT-15 cross-reacts to Zebrafish2
Application References(PubMed link indicates BioLegend citation): Segre CV, et al, 2016. mAbs. 8: 37-42 Loponte S, et al, 2016. Sci Rep, 6: 30213.
RRID: AB_2801113 (BioLegend Cat. No. 611003) AB_2801114 (BioLegend Cat. No. 611004)
Structure: HDAC1 is a 482 amino acid protein with a predicted molecular weight of 55 kD.
Distribution: Nucleus/Ubiquitously expressed
Function: Phosphorylation of HDAC1 Ser406 results in reduced deactylase activity of the enzyme.
Biology Area: Cell Biology, Cell Cycle/DNA Replication, Chromatin Remodeling/Epigenetics
Molecular Family: Nuclear Markers, Phospho-Proteins
Antigen References: Segre CV, et al, 2016. mAbs. 8: 37-42. Loponte S, et al, 2016. Sci Rep, 6: 30213.
Gene ID: 3065
UniProt: View information about HDAC1 Phospho Ser406 on UniProt.org
Clone: BT-15
Regulatory Status: RUO
Other Names: Histone Deactylase 1, RPD3L1
Isotype: Mouse IgG2b, κ
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.