Biolegend, 611252, Purified anti-TIF1γ/TRIM33 Antibody, 100μg

TIF1γ/TRIM33, belonging to the tripartite motif-containing (TIF) protein family, is a multifunctional protein that regulates hematopoiesis, DNA repair, mitosis, and transcription elongation. Just as with other TIF/TRIM family members, TIF1γ/TRIM33 contains a conservative RBCC/TRIM motif at the N terminus and a PHD/ bromodomain at the C terminus.  TIF1γ/TRIM33 suppresses the TGF-beta/BMP signaling pathway via monoubiquitylation of SMAD4 or the binding of phosphorylated SMAD2/3. Through its E3 ubiquitin ligase function, TIF1γ/TRIM33 decreases nuclear beta-catenin expression and suppresses tumorigenesis and tumor cell proliferation. TIF1γ/TRIM33’s tumor suppressive properties have been observed in hepatocellular carcinoma, chronic myelomonocytic leukemia, and pancreatic cancer. It has also been observed to reduce the stability of HIV-1 viral integrase to restrict HIV-1 infection.
100μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: E. coli derived recombinant fragment of human TIF1γ/TRIM33
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested ICC - Verified
Recommended Usage: Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.25 - 1.0 µg/mL. For immunocytochemistry, a concentration range of 1.25 - 5.0 μg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: This product is not recommended for use in IHC-P. For immunocytochemistry staining, the suggested fixation method is 4% paraformaldehyde.
RRID: AB_2936736 (BioLegend Cat. No. 611251) AB_2936736 (BioLegend Cat. No. 611252)
Structure: TIF1γ/TRIM33 is a 1127 amino acid protein with an expected molecular weight of 123 kD. It contains a RING domain, two B-box domains, and a coiled-coil domain (RBCC) at the N terminus; a plant homeodomain (PHD) and a bromodomain at the C terminus.
Distribution: Ubiquitous/ Mainly nuclear, some cytosolic
Function: Acts as E3 ubiquitin ligase to ubiquitylate target proteins, transcription inhibitor of TGF-beta/BMP pathway
Interaction: Homooligomer and heterooligomer with TRIM24 and TRIM28; interacts with SMAD2, SMAD3, and SMAD4.
Biology Area: Cell Cycle/DNA Replication, DNA Repair/Replication, Protein Trafficking and Clearance, Signal Transduction, Transcription Factors, Ubiquitin/Protein Degradation
Molecular Family: Enzymes and Regulators, Ligases, Tumor Suppressors
Antigen References: Xue J, et al. 2015. Nat Commun. 6:6156. Herquel B, et al. 2011. Proc Natl Acad Sci U. S. A. 108:8212-7. Ali H, et al. 2019. Nat Commun. 10:926.
Gene ID: 51592
UniProt: View information about TIF1gamma/TRIM33 on UniProt.org
Clone: 3H7-C8-B9
Regulatory Status: RUO
Other Names: Tripartite Motif Containing 33,TIF1G,RFG7.TIFGAMMA,TF1G,PTC7,E3 Ubiquitin-Protein Ligase,RING-Type E3 Ubiquitin Transferase,RET-Fused Gene 7,KIAA1113,Ectodermin,Transcription intermediary factor 1-gamma
Isotype: Mouse IgG1, κ