Biolegend, 611652, Purified anti-CREB1 Phospho (Ser133) Antibody, 200microl

Cyclic AMP (cAMP)-response element-binding protein 1 (CREB1) is a phosphorylation-dependent transcription factor that binds to the cAMP response element (CRE) in response to certain stimuli. CREB1 contains a basic leucine zipper (bZIP) DNA-binding domain, 1 dimerization domain, and 9 serine residues in 1 kinase inducible domain phosphorylated by multiple kinases, including Ca2+/CaM-dependent kinase (CaMK) II and IV, protein kinase A (PKA), protein kinase C (PKC), mitogen/stress-activated kinase (MSK), ribosomal S6 kinase (RSK), AKT, and MAPKAP kinase 2 (MK2). Both the protein levels and activation of CREB1 are vital for maintaining cellular homeostasis in physiological and pathological conditions. Phosphorylation of CREB1 at Ser133 promotes the recruitment of co-activator proteins CBP and p300, which has been proposed to increase transcription of CREB-dependent genes. Ser133 can also be phosphorylated by other proteins such as MSK1/2 in response to the activation of MAPK signaling.
200microl
Verified Reactivity: Human
Antibody Type: Polyclonal
Host Species: Rabbit
Immunogen: Synthetic peptide of human CREB phosphorylated at Ser133
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 1:2000-1:3000 dilution. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: The CREB1 peptide phosphorylated at Serine 133 used for immunization has 100% homology with ATF-1 sequence phosphorylated at serine 63. Therefore, this clone will detect both phosphorylated proteins if present in testing sample.
RRID: AB_3083400 (BioLegend Cat. No. 611651) AB_3083400 (BioLegend Cat. No. 611652)
Structure: CREB1 contains 327 amino acids at a predicted molecular weight at 43 kD
Distribution: Ubiquitously expressed/Nucleus
Function: Under physiological conditions, CREB1 maintains cellular survival, proliferation, anti-inflammatory immune responses and modulates the development of the nervous system by serving a neuroprotective role. Under pathological conditions, increased levels of CREB1 is associated with acute lymphoblastic leukemia (ALL), acute myeloid leukemia (AML), Hodgkin’s lymphoma, chronic lymphatic leukemia (CLL), melanoma, hepatocellular, renal cell, ovarian, prostate, lung, gastric, esophageal, pancreatic, and breast tumors. CREB1 is also involved in the aging process by regulating the downstream signals of mitochondria dysfunction.
Interaction: CREB1 interacts with CREBBP, CRTC2, EP300, CRTC1, JUN, AKT1, CAMK4, FOS, RPS6KA1, RPS6KA3, CaMKII, CaMKIV, PKA, PKC, MSK, RSK, AKT, and MK2.
Cell Type: B cells, Endothelial cells, Fibroblasts, Granulocytes, Macrophages, NK cells, T cells
Biology Area: Cell Biology, Transcription Factors
Antigen References: Andre S, et al. 2020. Cell Mol Life Sci. 77:4049-4067. Pei-Hsuan L, et al. 2020. Diagnostics. 10:295. Kensuke S, et al. 2011. J Neurochem. 116:1-9. Andy YW, et al. 2010. J Immunol. 185:6413-6419. Arnould T, et al. 2002. EMBO J. 21:53-63. Shaista N, et al. 2014. Biochem J. 458:469-79.
Gene ID: 1385
UniProt: View information about CREB1 Phospho Ser133 on UniProt.org
Clone: Poly22A03
Regulatory Status: RUO
Other Names: Cyclic AMP-Responsive Element-Binding Protein 1 (Serine 133), CAMP Responsive Element Binding Protein 1
Isotype: Rabbit Polyclonal IgG