Biolegend, 612004, Alexa Fluor® 647 anti-FOXA1 Antibody, 100μg

FOXA1 is a transcriptional activator that is required for tissue-specific differentiation during embryonic development. It also functions as a coactivator with nuclear hormone receptors, including ESR1, and has been shown to play a major role in driving estrogen receptor positive breast cancers.
100μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Full length recombinant human FOXA1
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: ICFC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by intracellular flow cytometry using our True-Nuclear™ Transcription Factor Staining Protocol. For flow cytometric staining, the suggested use of this reagent is ≤ 5 µl per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses
Excitation Laser: Red Laser (633 nm)
Application Notes: When using this clone for western blot, we highly recommend using lysate that has not been subjected to any freeze-thaw cycles.
Additional Product Notes: NOTE: For flow cytometric staining with this clone, True-Nuclear™ Transcription Factor Buffer Set (Cat. No. 424401) offers improved staining and is highly recommended.
Application References(PubMed link indicates BioLegend citation): Besenard V, et al. 2004. Gene Expr Patterns. 5:193-208. (IHC-P)
RRID: AB_2801117 (BioLegend Cat. No. 612003) AB_2801118 (BioLegend Cat. No. 612004)
Structure: FOXA1 is a 472 amino acid protein with a predicted molecular weight of 49 kD.
Distribution: Nucleus/Early endoderm derived cells
Function: Transcriptional activator
Interaction: FOXA2, NKX2-1, Histones H3 and H4
Biology Area: Cell Biology, Transcription Factors
Molecular Family: Nuclear Markers
Gene ID: 3169
UniProt: View information about FOXA1 on UniProt.org
Clone: 2F83
Regulatory Status: RUO
Other Names: Forkhead box protein A1, HNF3α, Hepatic nuclear factor 3 α
Isotype: Mouse IgG1, κ