Biolegend, 612158, Purified anti-human BMP-4 Antibody, 100μg

Bone morphogenetic proteins (BMPs) are multi-functional growth factors that belong to the transforming growth factor beta (TGFbeta) superfamily. BMPs play a key role in embryonic development, especially during heart, neural and cartilage development. Around 20 BMP family members have been identified and characterized. BMPs signal through serine/threonine kinase receptors, composed of type I and II subtypes. Four type I receptors have been identified: type IA and IB BMP receptors, type IA activin receptor, and activin receptor-like kinase I. Three type II receptors have also been recognized: type II BMP receptor and type II / IIB activin receptors. The BMP-4 gene is conserved across human, chimpanzee, Rhesus monkey, dog, cow, mouse, rat, and zebrafish species. BMP-2 and BMP-4 are highly similar, with a 92% amino acid sequence identity. BMP-4 is required for mesoderm formation and patterning in mice, and BMP-4 knock-out mice are embryonic-lethal. BMP-4 has an important role in postnatal tooth cytodifferentiation. Overexpression of BMP-4 has been associated with fibrodysplasia ossificans progressiva (FOP). Overexpression of BMP-4 has also been associated with cancer, including melanoma, hepatocellular carcinoma, adenocarcinoma and colorectal cancer cell lines. This overexpression can promote migratory and invasive properties of cancer cells.
100μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Human Recombinant BMP-4 Protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: Direct ELISA - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by Direct ELISA. The optimal concentration of this reagent for the specific application and the experiment design should be established by the end-user.
Additional Product Notes: This product is intended for sandwich-based immunoassay development on various assay platforms requiring antibody pairs. This antibody (clone A20137E) could function as a capture antibody when paired with biotin anti-human BMP-4 antibody (Cat No. 612159) (Clone A22044E) as the detection antibody and using recombinant Human BMP-4 (carrier-free) (Cat Nos. 795604, 795606, 591201, 595202) protein as the assay standard.
RRID: AB_3720585 (BioLegend Cat. No. 612158)
Structure: Disulfide-linked homodimer
Distribution: Osteoblast, odontoblast, ameloblasts, megakaryocytes, endothelial cells. Lymphoblastoid cell lines from patients with fibrodysplasia ossificans progressiva.
Function: Neural Stem Cells, mesenchymal stem cells, hematopoietic stem and progenitors, embryonic stem cells
Interaction: Chondroblast, osteoblast, retinoblastoma cells
Ligand/Receptor: Type I receptors BMPR-IA (ALK-3) and BMPR-IB (ALK-6)
Cell Type: Astrocytes, Embryonic Stem Cells, Mesenchymal Stem Cells, Oligodendrocytes
Biology Area: Cardiovascular Biology, Cell Biology, Immuno-Oncology, Neurodegeneration, Stem Cells
Molecular Family: Growth Factors
Antigen References: Zhong Z, et al. 2022. Bio Protoc. 12: . PubMed Mao QQ, et al. 2024. Exp Ther Med. 74:27. PubMed Chen WJ, et al. 2024. Cell Death Discov. 468:10. PubMed
Gene ID: 652
UniProt: View information about BMP-4 on UniProt.org
Clone: A20137E
Regulatory Status: RUO
Other Names: BMP-4, Bone morphogenetic protein 4, Bone morphogenetic protein 2B, BMP2B, BMP2
Isotype: Mouse IgG2b, κ