Biolegend, 612301, Purified anti-Cytochrome c Antibody, 25μg

Cytochrome c is a 15 kD protein found in the mitochondrial intermembrane space with a heme-binding domain. Cytochrome c is a component of the electron transport chain; the heme group transfers electrons from cytochrome b-c1 complex to cytochrome oxidase complex. Cytochrome c initiates apoptosis by release to cytoplasm and binding Apaf-1 which activates procaspase 9. Cytochrome c interacts with the cytochrome b-c1 complex, cytochrome oxidase complex, heme, Apaf-1, and Caspase 9 proteins. The 6H2.B4 monoclonal antibody recognizes human, mouse, and rat cytochrome-c and has been shown to be useful for intracellular flow cytometric staining, Western blotting, immunoprecipitation, and immunofluorescence staining.
25μg
Verified Reactivity: Mouse, Rat, Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Rat cyt c-OVA
Formulation: This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide. Final antibody concentration is 0.5 mg/ml.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: Upon receipt, store between 2°C and 8°C.
Application: IP - Quality testedICC - VerifiedICFC - Reported in the literature, not verified in house
Recommended Usage: Each lot of this antibody is quality control tested by immunoprecipitation. This antibody can be used at 2.0 - 4.0 µg /1 x107 cell equivalents for immunoprecipitation. For Western blot application, the suggested use of this reagent is 0.5 µg per ml (1:1000 dilution). For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: Additional reported applications (for the relevant formats) include: intracellular flow cytometry5, immunofluorescence microscopy3,5, immunoprecipitation4, and immunocytochemistry5.
Application References(PubMed link indicates BioLegend citation): Goshorn SC, et al. 1991. J. Biol. Chem. 266:2134. Jemmerson R, et al. 1991. Eur. J. Immunol. 21:143. Chandra D, et al. 2002. J. Biol. Chem. 277:50842. (IF) Semenkova L, et al. 2003. Eur. J. Biochem. 270:4388. (IP) Shih S-F, et al. 2001. J. Biol. Chem. 276:21870. (ICFC ICC IF) She P, et al. 2011. Am J. Physiol Endcorinol Metab.301:E49. PubMed McGuire, KA., et al. 2011. J. Virol 85:10806. PubMed
Product Citations: Cao YG, et al. 2022. Cell Host Microbe. 1295:30. PubMed Wang Z, et al. 2020. Theranostics. 5.401388889. PubMed Kruppa AJ et al. 2018. Developmental cell. 44(4):484-499 . PubMed She P, et al. 2011. Am J Physiol Endocrinol Metab. 301:E49. PubMed McGuire K, et al. 2011. J Virol. 85:10806. PubMed Rana PS, et al. 2020. J Cell Sci. 133:00:00. PubMed Ryan TA, et al. 2020. EMBO J. 39:e102539. PubMed Ponsford AH, et al. 2020. Autophagy. :1. PubMed Cserép C et al. 2018. eNeuro. 5(1) pii: ENEURO. PubMed
RRID: AB_315774 (BioLegend Cat. No. 612301) AB_315775 (BioLegend Cat. No. 612302)
Structure: Heme binding domain; 15 kD
Distribution: Mitochondrial intermembrane space
Function: Component of electron transport chain; heme group transfers electrons from cytochrome b-c1 complex to cytochrome oxidase complex. Initiates apoptosis by release to cytoplasm and binding Apaf-1 which activates procaspase 9
Interaction: Cytochrome b-c1 complex, cytochrome oxidase complex, heme, Apaf-1, Casp9
Biology Area: Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Mitochondrial Function, Neuroscience, Neuroscience Cell Markers
Molecular Family: Mitochondrial Markers
Antigen References: 1. Liu X, et al. 1996. Cell. 86:147. 2. Li P, et al. 1997. Cell. 91:479. 3. Zhang Z, et al. 2003. Gene 312:61. 4. Ferguson H, et al. 2003. J. Biol. Chem. 278:45793.
Gene ID: 1355
UniProt: View information about Cytochrome c on UniProt.org
Clone: 6H2.B4
Regulatory Status: RUO
Other Names: Cyt c
Isotype: Mouse IgG1, κ