Biolegend, 612752, Purified anti-mouse CCL24 Antibody, 100μg

CLL24 was initially cloned from a library of activated macrophages, and using human eosinophils, it was shown that it binds to the receptor of eotaxin-1 (CCL11) and MCP-4 (CCL13). In addition, CCL24 shares the CCR3 receptor with CCL26, CCL5, and CCL7. Eotaxin/CCR3 expression has been studied extensively in the pathogenesis of asthma and allergy. In addition, the eotaxin/CCR3 axis has been associated to additional inflammatory and autoimmune disorders including inflammatory bowel disease, multiple sclerosis, eosinophilic esophagitis, and rheumatoid arthritis. CCL24 has also been associated with neovascular age related macular degeneration.
100μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Recombinant Mouse CCL24 Protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: Direct ELISA - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by Direct ELISA. The optimal concentration of this reagent for the specific application and the experiment design should be established by the end-user.
Additional Product Notes: This production is intended for immunoassay development on various assay platforms requiring antibody pairs. This antibody (clone A18110C) could function as a capture antibody when paired with Biotin anti-mouse CCL24 antibody (Cat. No. 612153) (clone A18110F) as the detection antibody and using Recombinant Mouse CCL24 (Eotaxin-2) protein (Cat. No. 585102) as the assay standard.
RRID: AB_2941609 (BioLegend Cat. No. 612752)
Structure: Chemokine
Distribution: CCL24 is expressed by epithelial cells, airway smooth muscle cells, and vascular endothelial cells. In addition, eotaxins are produced by cells isolated by bronchial alveolar lavage, including macrophages, monocytes, basophils, eosinophils, dendritic cells, and lymphocytes.
Function: CCL24 chemoattracts CCR3 bearing cells such as eosinophils, mast cells, basophils, and Th2 cells that play a role in allergic disorders. IL-4 and IL-13 induce CCL11 and CCL24 in esophagus and colonic epithelial cell, and IL-13 induces the production of CCL-24 in dendritic cells and alveolar macrophages. In addition, CCL24 is induced by IL-33 in lungs, and by IL-4 and LPS in monocytes and macrophages. The expression of CCL24 by alveolar epithelial cells requires histamine and its receptor H2R.
Interaction: Eosinophils, mast cells, Th2 lymphocytes, basophils, human dendritic cells, synovial tissue macrophages, lung epithelial cells, and airway smooth muscle cells
Ligand/Receptor: CCR3
Biology Area: Cell Biology, Signal Transduction
Molecular Family: Cytokines/Chemokines
Antigen References: Patel VP, et al. 1997. J Exp. Med. 185:1163-72. Beaulieu S, et al. 2002. J Immunol. 169:2925-36. Watanabe K, et al. 2002. J Immunol. 168:1911-8. Ablin JN, et al. 2010. Clin Exp Immunol. 161:276-83. Manousou P, et al. 2010. Clin Exp Immunol. 162:337-47. Stolarski B, et al. 2010. J Immunol. 185:3472-80. Crapster-Pregont M, et al. 2012. J Allergy Clin Immunol. 129:1621-7. Swartzendruber JA, et al. 2012. J Immunol. 188:536-40.
Gene ID: 56221
UniProt: View information about CCL24 on UniProt.org
Clone: A18110C
Regulatory Status: RUO
Other Names: MPIF-2
Isotype: Rat IgG1, κ