Biolegend, 614002, Purified anti-GATA2 Antibody, 100μg

GATA2 is a member of the GATA transription factor family. Mutations in GATA2 lead to impaired development and differentiation of hematopoietic  lineages, including dendritic cells, monocytes, B cells, and natural killer cells. Over the course of differentiation, GATA2 activates expression of the functionally distinct GATA1; promoter-occupied GATA2 is replaced/exhanged at certain promoters with the GATA1 transcription factor, a process known as the GATA switch.
100μg
Verified Reactivity: Human, Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Recombinant partial human GATA2 protein corresponding to amino acids 1-250
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested ICFC - Verified
Recommended Usage: Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.1 - 1.0 µg/mL. For intracellular flow cytometry using our True-Nuclear™ Transcription Factor Staining Protocol, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
RRID: AB_2810661 (BioLegend Cat. No. 614001) AB_2810662 (BioLegend Cat. No. 614002)
Structure: GATA2 is a 480 amino acid protein with a predicted molecular weight of 51 kD.
Distribution: Widely expressed/nucleus
Function: Transcriptional activator/Regulates developmental processes in hematopoietic cells
Cell Type: Lymphocytes
Biology Area: Cell Biology, Stem Cells, Transcription Factors
Molecular Family: Nuclear Markers
Antigen References: Orkin SH. 1992. Blood. 80: 575. Bresnick EH, et al. 2010. J. Biol. Chem. 285: 31087. Collin M, et al. 2011. Nat. Rev. Immunol. 11: 575. Doré LC, et al. 2012. Blood. 119: 3724.
Gene ID: 2624
UniProt: View information about GATA2 on UniProt.org
Clone: W17242A
Regulatory Status: RUO
Other Names: GATA Binding Protein 2, IMD21, NFE1B, DCML
Isotype: Rat IgG2b, κ