Biolegend, 614302, Purified anti-PARP Antibody, 100μg

PARP (Poly (ADP-ribose) polymerase) is a 113 kD nuclear protein that can exist as a homo- or hetero-dimer. This protein acts as a molecular "nick sensor" and functions in base excision repair, poly(ADP-ribosyl)ation of acceptor proteins involved in chromatin architecture and DNA metabolism and participates in protein modification to enhance or repress transcription. PARP is ribosylated by PARP2 and is a target for caspase cleavage during apoptosis. PARP interacts with proteins in the base excision repair complex containing at least XRCC1, PARP2, POLB and LIG3. In addition PARP forms heterodimers with PARP2, and interacts with PARP3. The 5A5 monoclonal antibody recognizes the N-terminal region of human and mouse PARP and has been shown to be useful for Western blotting and immunofluorescence staining.
100μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Recombinant (partial), N-terminal 2/3 sequence of PARP
Formulation: This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide. Final antibody concentration is 0.5 mg/ml.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: Upon receipt, store undiluted between 2°C and 8°C.
Application: WB - Quality tested KO/KD-WB, ICC, IHC-P - Verified
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 1.0 µg/mL (1:500-1:1000 dilution). For immunocytochemistry, a concentration range of 1 - 5 μg/mL is recommended. For immunohistochemistry, a concentration range of 5 - 10 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: This antibody recognizes N-terminal short cleaved PARP after Staurosporine treatment at around 24kDa.
Application References(PubMed link indicates BioLegend citation): Bogiatzi SI, et al. 2012. J Allergy Clin Immunol. 130:233. PubMed. Gu Y, et al. 2013. Acta Biochim Biophys Sin. PubMed
Product Citations: Aggarwal M, et al. 2023. Cancers (Basel). 15: . PubMed Nakaya M, et al. 2017. J Clin Invest. 127:383. PubMed Wang F, et al. 2020. Int J Oncol. 57:707. PubMed Bogiatzi S, et al. 2012. J Allergy Clin Immunol. 130:233. PubMed Gu Y, et al. 2013. Acta Biochim Biophys Sin. 45:904. PubMed
RRID: AB_2160595 (BioLegend Cat. No. 614301) AB_2299318 (BioLegend Cat. No. 614302)
Structure: PARP family, BRCT domain, homo- or hetero-dimer; 113 kD
Distribution: Nuclear
Function: Molecular "nick sensor"; base excision repair, catalyzes poly(ADP-ribosyl)ation of acceptor proteins involved in chromatin architecture, DNA metabolism; protein modification may enhance or repress transcription
Interaction: Component of a base excision repair complex containing at least XRCC1, PARP2, POLB and LIG3. Heterodimerizes with PARP2, interacts with PARP3, modifies TATA-BP, YY1, Sp1, NF-B, p53 and others
Modification: Ribosylation by PARP2
Biology Area: Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, DNA Repair/Replication, Transcription Factors
Molecular Family: Nuclear Markers
Antigen References: 1. Cherney B, et al. 1987. P. Natl. Acad. Sci. USA 84:8370. 2. Ikejima M, et al. 1990. J. Biol. Chem. 265:21907. 3. Ying W, et al. 2001. P. Natl. Acad. Sci. USA 98:12227. 4. Noel G, et al. 2003. BMC Cell Biol. 4:7.
Regulation: Poly(ADP-ribose) glycohydrolase removes ribose chains, allows quick, transient ribosylation of proteins
Gene ID: 142
UniProt: View information about PARP on UniProt.org
Clone: 5A5
Regulatory Status: RUO
Other Names: Poly (ADP-ribose) polymerase
Isotype: Mouse IgG1, κ