Biolegend, 614452, Purified anti-RPS27A Antibody, 100μg

Ubiquitin is a small (8.5 kD) regulatory protein that is ubiquitously expressed in tissues of eukaryotic organisms. There are four genes in the human genome that produce ubiquitin: UBB, UBC, UBA52, and RPS27A. UBA52 and RPS27A genes code for a single copy of ubiquitin fused to the ribosomal proteins L40 and S27a, respectively. The UBB and UBC genes code for polyubiquitin precursor proteins. Ubiquitination is a post-translational modification where a ubiquitin subunit is attached to a protein. Addition of ubiquitin can signal for degradation via the proteasome, alter cellular location, promote or prevent protein interactions, or affect activity. Ubiquitination is carried out stepwise by ubiquitin-activating enzymes (E1s), ubiquitin-conjugating enzymes (E2s), and ubiquitin ligases (E3s), respectively. The cascade results in the binding of ubiquitin to lysine residues on the protein substrate via an isopeptide bond, cysteine residues through a thioester bond, serine and threonine residues through an ester bond, or the amino group of the protein's N-terminus via a peptide bond. Proteins can be modified either by a single ubiquitin unit (monoubiquitination) or a chain of ubiquitin molecules (polyubiquitination). Proteins destined for degradation by the proteasome are usually ubiquitinated on lysine residues K48 and K29, while other polyubiquitinations (e.g. on K63, K11, K6) and monoubiquitinations may regulate processes such as endocytic trafficking, inflammation, translation and DNA repair. A frameshift mutation in ubiquitin B can result in a truncated peptide missing the C-terminal glycine. This abnormal peptide, known as UBB+1, has been shown to accumulate selectively in Alzheimer's disease and other tauopathies.
100μg
Verified Reactivity: Human, Mouse, Rat
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Recombinant fragment of human RPS27A
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: IHC-P - Quality tested WB - Verified
Recommended Usage: Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 1.0 - 5.0 µg/mL is suggested. For western blotting, the suggested use of this reagent is 0.125 - 1.0 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: This product is not recommended for use in ICC. For immunohistochemistry, antigen retrieval with sodium citrate is recommended. Clone A18156E is equivalent to BioLegend’s clone P4G7 which detects ubiquitin and ubiquitinylated proteins. The ubiquitin protein is extremely well conserved and thus the antibodies have extensive species cross-reactivity from yeast to human.
RRID: AB_3068138 (BioLegend Cat. No. 614451) AB_3068138 (BioLegend Cat. No. 614452)
Structure: RPS27A is a 156 amino acid S27a ribosomal protein fused with a single copy of ubiquitin.
Distribution: Found in almost all tissues of eukaryotic organisms
Function: Regulatory protein
Biology Area: Cell Biology, Neurodegeneration, Neuroscience, Neuroscience Cell Markers, Protein Trafficking and Clearance, Signal Transduction
Molecular Family: Autophagosome Markers
Antigen References: Hatanaka H, et al. 2009. J. Biol. Chem. 284:15448-57. Sarkari F, et al. 2013. J. Biol. Chem. 288:16975-16985.
Gene ID: 6233
UniProt: View information about RPS27A on UniProt.org
Clone: A18156E
Regulatory Status: RUO
Other Names: Polyubiquitin-C, Polyubiquitin B, RPS 27A, RPS27A, UBA 52, UBA 80, UBA52, UBA80, UBB, UBC, UBCEP 1, UBCEP 2, UBCEP1, UBCEP2, Ubiquitin, ubiquitin B
Isotype: Mouse IgG2a, κ