Biolegend, 614652, Purified anti-APCS (PTX2) Antibody, 100μg

APCS (amyloid P component, serum), also known as pentraxin 2 (PTX2), is a member of the pentraxin family, which has a non-covalently linked pentameric organization. This protein is characterized by its calcium-dependent ligand binding and distinctive flattened β-jellyroll structure similar to legume lectins. Human APCS shares a 79% amino acid homology with its mouse counterpart. APCS is secreted by hepatocytes as part of the acute phase protein synthesis in response to infections. Upon binding to its ligands, APCS is involved in the activation of classical complement cascade. In a mouse model, APCS was shown to downregulate acute inflammation accompanying experimental autoimmune encephalomyelitis by regulating T cell activity. In addition to its role in regulating immune response, APCS is found to be a component of amyloid deposits which have the ability of stabilizing amyloid fibrils and protecting them from proteolytic degradation. It is postulated that APCS may play a role in Alzheimer's disease and other amyloidosis. Interestingly, APCS is also identified as a component of basement membrane proteins and is able to bind laminin, collagen IV, P-selectin, and fibronectin in a Ca2+ dependent manner. In addition, APCS is known to specifically localize in human atherosclerotic lesions, which suggests that it may play a role in atherogenesis. Functional studies revealed that APCS binds to lipoproteins, specifically to HDL, but not LDL.
100μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Recombinant fragment of human APCS
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: IHC-P - Quality testedWB - Verified
Recommended Usage: Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 1.0 - 10.0 µg/mL is suggested. For western blotting, the suggested use of this reagent is 0.1 - 1.0 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.
RRID: AB_2941618 (BioLegend Cat. No. 614651) AB_2941618 (BioLegend Cat. No. 614652)
Structure: Monomer
Distribution: Liver, serum and urine
Function: APCS recognizes microbial pathogens and activates the classical complement pathway; it binds to Fcγ receptors and opsonizes particles for phagocytosis
Interaction: Cells expressing Fcγ receptors
Ligand/Receptor: FcγR (CD16, CD32, and CD64), collagen IV, laminin, HDL, VLDL, fibronectin, CRP, and CD62L
Biology Area: Complement, Neurodegeneration, Neuroinflammation
Molecular Family: APP/β-Amyloid, APP/Aβ Degradation
Antigen References: Tennent GA, et al. 1995. Proc Natl Acad Sci USA. 92:4299-303. Li XA, et al. 1998. Biochem Biophys Res Commun. 244:249-52. Zahedi K. 1996. J Biol Chem. 271:14897-902. Pepys M, et al. 2002. Nature. 417:254-9. Stibenz D, et al. 2006. Eur J Immunol. 36:446-56. Lu J, et al. 2008. Nature. 456:989-92.
Gene ID: 325
UniProt: View information about APCS on UniProt.org
Clone: W19147A
Regulatory Status: RUO
Isotype: Rat IgG2a, κ