Biolegend, 614751, Purified anti-PGR Antibody, 25μg

PGR (Progesterone receptor) is a nuclear receptor, a type of receptor protein that directly interacts with DNA to affect gene expression upon ligand binding. PGR is activated by the hormone progesterone, a steroid and sex hormone. Widely expressed in female reproductive tissues, activation of PGR is critical for the establishment and maintenance of pregnancy and in the development of the mammary glands. PGR is also expressed in the central nervous system, modulating innervation by dopaminergic neurons. Overexpression of PGR is often found in breast, ovarian and uterine cancers, and PGR is one of the three major receptors assessed when classifying breast tumors.
25μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Recombinant human progesterone receptor
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: IHC-P - Quality tested ICC, WB - Verified
Recommended Usage: Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 1.0 - 10.0 µg/mL is suggested. For immunocytochemistry, a concentration of 1.25 μg/mL is recommended. For western blotting, the suggested use of this reagent is 0.25 - 1.0 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: This product does not recognize mouse progesterone receptor in Western blot. This product only recognizes PGR Isoform B. For immunocytochemistry, fixation with Fixation Buffer (Cat. No. 420801) followed by permeabilization with 0.5% Triton-X is recommended. Fixation with Fixation Buffer followed by permeabilization with 100% methanol, or fixation with 100% methanol are not recommended.
RRID: AB_2941619 (BioLegend Cat. No. 614751) AB_2941619 (BioLegend Cat. No. 614752)
Structure: PGR has two major isoforms. Isoform A is a 769 amino acid protein with a predicted molecular weight of 82.3 kD. Isoform B is a 933 amino acid protein with a predicted molecular weight of 99.0 kD.
Distribution: Nucleus
Function: Nuclear receptor, transcription factor
Interaction: Upon ligand binding, PGR interacts directly with genomic DNA
Ligand/Receptor: Progesterone
Cell Type: Dopaminergic Neurons, Hematopoietic stem and progenitors, Neurons
Biology Area: Cell Cycle/DNA Replication, Neuroscience, Stem Cells, Transcription Factors
Molecular Family: Hormones, Steroid Receptors/Nuclear Receptors
Antigen References: Grimm SL, et al. 2016. J Mol Biol. 428:3831-49. Yip CH, et al. 2014. Future Oncol. 10:2293-301. Cope DI and Mosivais D. 2022. Cells. 11:1474.
Gene ID: 5241
UniProt: View information about PGR on UniProt.org
Clone: BSB-2
Regulatory Status: RUO
Other Names: Progesterone receptor (PGR), nuclear receptor subfamily 3, group C, member 3 (NR3C3)
Isotype: Mouse IgG1, κ