Biolegend, 614952, Purified anti-CD73 Antibody, 100μg

CD73 (cluster of differentiation 73), also known as ecto-5’-nucleotidase (e5NT), is a membrane-bound glycoprotein and ectoenzyme. CD73 catalyzes the hydrolysis of AMP generated by CD39 to adenosine to activate adenosine signaling. This role makes CD73 crucial to the regulation of purinergic signaling and immune homeostasis and response. CD73 also serves a variety of physiological functions, including lymphocyte binding, epithelial ion transport, ischemic preconditioning, tissue-barrier function, T cell activation, and tissue homeostasis. Overexpressed in several cancers and implicated in tumor progression, metastasis, and the impairment of anti-tumor immune response via adenosine receptor (AR) signaling, CD73 is a target of interest in cancer therapy. It is also a potential immunotherapy target for the treatment of pain, inflammation, and hypoxia.
100μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Synthetic peptide corresponding to human CD73
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested ICC, FC - Verified
Recommended Usage: Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.25 - 1.0 µg/mL. For immunocytochemistry, a concentration range of 1.25 - 5.0 μg/mL is recommended. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: This clone is not mouse or rat reactive. This clone is not suitable for use in immunohistochemistry. For use in ICC, we recommend fixation with ice-cold methanol or fixation with PFA followed by permeabilization with ice-cold methanol.
RRID: AB_2941621 (BioLegend Cat. No. 614951) AB_2941621 (BioLegend Cat. No. 614952)
Structure: CD73 is a 574 amino acid protein with a predicted molecular weight of 63 kD.
Distribution: Cytoplasmic and membranous expression in most tissues
Function: Hydrolase, metabolic protein, CD marker, enzyme
Interaction: AP-2, SMAD, SP-1, CD39, GSDMD
Ligand/Receptor: Metal-binding, nucleotide-binding, zinc
Cell Type: Lymphocytes, T cells
Biology Area: Cell Biology
Molecular Family: CD Molecules, Enzymes and Regulators, Innate Immune Signaling
Antigen References: Knapp K, et al. 2012. Structure. 20:2161-73. Minor M, et al. 2019. Am J Physiol Cell Physiol. 317:C1079-C1092. Yu M, et al. 2020. Nat Commun. 11:515.
Gene ID: 4907
UniProt: View information about CD73 on UniProt.org
Clone: AA60-E3-3
Regulatory Status: RUO
Other Names: NT5E, 5'-Nucleotidase Ecto, Ecto-5'-Nucleotidase, CALJA, ENT, NT5, EN, E5NT
Isotype: Mouse IgG1, κ