Biolegend, 615252, Purified anti-SP1 Antibody, 100μg

SP1 belongs to the transcription factor SP/KLF family, which contains three zinc finger domains that can bind to GC boxes and regulate transcription of a variety of molecules. SP1 regulates efferent gene transcription positively and negatively, participating broadly in cellular development and physiological functions as a generally expressed transcription factor. Post-translational modifications, including phosphorylation, glycosylation, acetylation, ubiquitylation, and SUMOylation, highly regulate SP1’s function and stability. SP1 is required to develop and differentiate early embryo, placenta, and sperm cells. SP1 is highly expressed in multiple cancers including breast, stomach, pancreas, lung, and brain. SP1 promotes tumor cell proliferation, inactivates cell death, and facilitates immune escape.
100μg
Verified Reactivity: Human, Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: E. coli derived recombinant fragment of human SP1
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested ICC, IHC-P - Verified
Recommended Usage: Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.25 - 1.0 µg/mL. For immunocytochemistry, a concentration range of 1.25 - 5.0 μg/mL is recommended. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 1.0 - 10.0 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
Additional Product Notes: The mouse cross-reactivity of this product has been verified by WB. For use in immunohistochemistry (IHC), antigen retrieval with Tris-EDTA pH 9.0 is recommended. For use in immunocytochemistry (ICC), paraformaldehyde fixation is recommended.
RRID: AB_3083401 (BioLegend Cat. No. 615251) AB_3083401 (BioLegend Cat. No. 615252)
Structure: SP1 is a 785 amino acid protein with an expected molecular weight of about 80.6 kD.
Distribution: Nuclear/Ubiquitous
Function: Binds to GC boxes to regulate transcriptional activity
Interaction: EP300, E2F1, RELA, HDACs, SMADs, BRCA1, BRCA2, RNF4, c-MYC, c-JUN, p53
Biology Area: Angiogenesis, Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Cell Cycle/DNA Replication, Cell Death, Cell Proliferation and Viability, Chromatin Remodeling/Epigenetics, DNA Repair/Replication, Immunology, Neurodegeneration, Signal Transduction, Transcription Factors
Molecular Family: Nuclear Markers
Antigen References: Saffer JD, et al. 1991. Mol Cell Biol. 11:2189-99. Thomas K, et al. 2007. Mol Cell Endocrinol. 270:1-7. Vizaino C, et al. 2015. Pharmacol Ther. 152:111-24. Beishline K, et al. 2015. FEBS J. 282:224-58.
Gene ID: 6667
UniProt: View information about SP1 on UniProt.org
Clone: W21043E
Regulatory Status: RUO
Other Names: Transcription factor sp1, TSFP1, Specificity Protein 1
Isotype: Rat IgG2a, κ