Biolegend, 615354, Alexa Fluor® 647 anti-IBA1 Antibody, 100tests

As a cytoplasmic scaffold protein, IBA1 contains Ca2+ binding EF-hand and PDZ interaction domains, which are important for mediating intracellular signaling complexes. IBA1, also known as AIF-1, was first observed in rodent microglial cells and has been shown to stain cells in both normal and pathological lesions in rodents. In addition to the central nervous system, IBA1 has also been shown to stain in macrophage subpopulations. In macrophages, expression of IBA1 promotes pro-inflammatory responses. In microglial cells, IBA1 is important in the modulation of synaptic activities and is upregulated in response to nerve damage.
100tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Synthetic peptide from human IBA1
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: ICFC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by intracellular flow cytometry using our Cyto-Fast™ Fix/Perm Buffer Set. For flow cytometric staining, the suggested use of this reagent is 5 µL per 106 cells in 100 µl volume. It is highly recommended that the reagent be titrated for optimal performance for each application.* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses
Excitation Laser: Red Laser (633 nm)
Application Notes: For use in intracellular flow cytometry (ICFC), it is recommended to fix/permeabilize with Cyto-Fast™ Fix/Perm Buffer Set (Cat. No. 426803).It is not recommended using this product in immunocytochemistry (ICC) or immunohistochemistry on FFPE tissues (IHC-P).This antibody clone does not detect mouse or rat IBA1 in intracellular flow cytometry (ICFC).
RRID: AB_3699062 (BioLegend Cat. No. 615353) AB_3699062 (BioLegend Cat. No. 615354)
Structure: IBA1 is a 147 amino acid protein with a predicted molecular weight of 17 kD.
Distribution: Expressed in microglia and macrophages
Function: Plays a role in inflammatory response and modulates synaptic activities
Interaction: Interacts with LCP1
Ligand/Receptor: Calcium
Cell Type: Lymphocytes, Macrophages, Microglia, T cells
Biology Area: Apoptosis/Tumor Suppressors/Cell Death, Cell Death, Immunology, Innate Immunity, Neuroinflammation, Neuroscience, Neuroscience Cell Markers, Synaptic Biology
Molecular Family: Innate Immune Signaling
Antigen References: Elizondo D. M. et al. 2017. Front Immunol. 8:1502. Mikkelsen HB, et al. 2017. Anat Rec (Hoboken). 300:1114-1122. Shapiro LA, et al. 2009. Brain Res. 1266:29-36.
Gene ID: 199
UniProt: View information about IBA1 on UniProt.org
Clone: A19290E
Regulatory Status: RUO
Other Names: IBA1, Allograft Inflammatory Factor 1, IRT-1, Interferon Gamma Responsive Transcript
Isotype: Mouse IgG2a, κ