Biolegend, 615551, Purified anti-JMJD6 Antibody, 25μg

Bifunctional arginine demethylase and lysyl hydroxylase, also known as Jumonji domain-containing protein 6 (JMJD6), is a multifunctional enzyme that plays a critical role in regulating transcription and translation. One of its primary roles is the regulation of RNA splicing, where it acts as a co-factor for RNA-binding proteins that modulate alternative splicing events. Additionally, JMJD6 can also directly modify mRNA, modulating its stability, processing and translation. Previously, JMJD6 had been proposed to act as a histone demethylase; however, this function is a matter of some dispute. JMJD6 has been shown to regulate immune responses, cell differentiation, and embryogenesis. Overall, JMJD6 plays an important role in coordinating different cellular pathways, and its dysregulation has been linked to various human diseases, including cancer and autoimmune disorders.
25μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Recombinant protein corresponding to the N-terminus of human JMJD6
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: IHC-P - Quality tested ICC, WB - Verified
Recommended Usage: Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 0.5 - 5.0 µg/mL is suggested. For immunocytochemistry, a concentration range of 1.25 - 5.0 μg/mL is recommended. For western blotting, the suggested use of this reagent is 0.1 - 1.0 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: This product only weakly recognizes mouse JMJD6 in Western Blot and is not recommended for that purpose For ICC, fixation/permeabilization with 4% PFA followed by 100% ice-cold methanol is recommended. Fixation/permeabilization with 10% ice-cold methanol or 4% PFA followed by 0.5% Triton-X is not recommended.
RRID: AB_3068229 (BioLegend Cat. No. 615551) AB_3068229 (BioLegend Cat. No. 615552)
Structure: JMJD6 is a 403 amino acid protein with a predicted molecular weight of 46.5 kD
Distribution: JMJD6 is found primarily in the nucleus
Function: Arginine demethylase, lysine hydroxylase
Ligand/Receptor: Originally identified as a receptor for phosphatidyl serine, this is no longer thought to be relevant to JMJD6 function
Biology Area: Angiogenesis, Chromatin Remodeling/Epigenetics
Molecular Family: Enzymes and Regulators
Antigen References: Hahn P, et al. 2010. PLoS One. 5:e13769. Kwok J, et al. 2017. Front Genet. 8:32. Bottger A, et al. 2015. Biochem J. 468:191-202. Wang K, et al. 2022. Front Immunol. 13:859893.
Gene ID: 23210
UniProt: View information about JMJD6 on UniProt.org
Clone: W20240B
Regulatory Status: RUO
Other Names: Bifunctional arginine demethylase and lysyl hydroxylase JMJD6, Jumonji domain-containing protein 6, phosphatidylserine receptor (PSR, PTDSR1)
Isotype: Rat IgG2b, κ