Biolegend, 615954, Alexa Fluor® 647 anti-Olig2 Antibody, 100μg

Olig2 is a transcription factor that plays a crucial role in the development and maintenance of the central nervous system. This transcription factor is a master regulator for oligodendrocyte cell specification. Olig2 activates the expression of myelin-associated genes in oligodendrocyte lineage cells, which are essential for proper neuronal function and transmission of nerve impulses. In addition to its role in oligodendrocyte differentiation and maturation, Olig2 also regulates the development and maintenance of motor neurons in the spinal cord and contributes to the proliferation and differentiation of neural stem cells. Dysregulation of Olig2 expression has been associated with several neurological disorders, including multiple sclerosis and gliomas, highlighting its importance in maintaining normal brain function. Being located on chromosome 21, dysregulation of Olig2 is also associated with Down Syndrome.
100μg
Verified Reactivity: Human, Rat
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Recombinant fragment of the N-terminus of mouse Olig2
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: ICFC - Quality tested ICC - Verified
Recommended Usage: Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. For immunocytochemistry, a concentration range of 2.5 - 10.0 μg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses
Excitation Laser: Red Laser (633 nm)
Additional Product Notes: For use in immunocytochemistry (ICC), it is recommended to fix/permeabilize with either of the following: - Fixation Buffer (Cat. No. 420801) with 0.5 % Triton X-100 - Fixation Buffer (Cat. No. 420801) with 100% ice-cold methanol For use in intracellular flow cytometry (ICFC), it is recommended to fixation/permeabilization with True-Phos™ Perm Buffer (Cat. No. 425401).
RRID: AB_3720495 (BioLegend Cat. No. 615953) AB_3720495 (BioLegend Cat. No. 615954)
Structure: Olig2 is a 323 amino acid protein with a predicted molecular weight of 32.38 kD.
Distribution: Nucleus
Function: Transcription factor
Cell Type: Neural Stem Cells, Oligodendrocytes
Biology Area: Cell Biology, Neuroscience, Neuroscience Cell Markers, Transcription Factors
Antigen References: Zhang K, et al. 2022. Nat Commun. 13:1423. Xu R, et al. 2019. Cell Stem Cell. 24:908-926. Sock E, Wegner M. 2021. Dev Neurobiol. 81:892-901. Szu J, et al. 2021. Front Neurosci. 15:659601.
Gene ID: 10215
UniProt: View information about Olig2 on UniProt.org
Clone: W21023A
Regulatory Status: RUO
Other Names: Oligodendrocyte transcription factor 2 (Olig2), Class B basic helix-loop-helix protein 1 (bHLHb1), Class E basic helix-loop-helix protein 19 (bHLHe19), Protein kinase C-binding protein 2 (PRKCBP2), RACK17, OLIGO2
Isotype: Rat IgG2b, κ